Abstract
WE STUDIED THREE DIFFERENT TRICEPIROS: (Don Santiago x Don Noé), (Cumé x Horovitz) and (Cumé x Don Noé). The tricepiro (Don Santiago x Don Noé) was obtained by crossing the triticale Don Santiago INTA (AABBRR, 2n = 6x = 42) with the trigopiro Don Noé INTA (AABBDDJJ, 2n = 8x = 56). The number of chromosomes for the F(1) was 2n = 49, the most frequent meiotic configuration being 14 bivalents and 21 univalents. The univalents were situated in the periphery of the equatorial plane, whereas the bivalents were located in the central zone. The chromatids in some of the univalents split when bivalents underwent reductional division in anaphase I. There were few laggard chromosomes or chromatids at this phase. The number of chromosomes (2n = 48-58) was high and variable, and the number of bivalents per cell (18-23) also high in F (3) individuals. In all F (8) tricepiros (Don Santiago x Don Noé), F (12) tricepiros (Cumé x Horovitz) and F (12) tricepiros (Cumé x Don Noé), the number of chromosomes (2n = 42) was the same, these retaining the rye genome, as demonstrated by GISH and FISH. These new synthesized allopolyploids constitute interesting models for investigating the evolutionary changes responsible for diploidization, and the chromosomal and genomic re-ordering that cannot be revealed in natural allopolyploids.
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Claims 10
Claim · model-written summary
The Don Santiago × Don Noé F1 had 49 chromosomes.
Verbatim
e0“The number of chromosomes for the F(1) was 2n = 49”
e1“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
The most frequent F1 meiotic configuration comprised 14 bivalents and 21 univalents.
Verbatim
e0“the most frequent meiotic configuration being 14 bivalents and 21 univalents”
e1“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
Univalents lay at the equatorial periphery while bivalents lay centrally.
Verbatim
e0“The univalents were situated in the periphery of the equatorial plane, whereas the bivalents were located in the central zone.”
e1“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
Some univalent chromatids split during bivalent reductional division in anaphase I.
Verbatim
e0“The chromatids in some of the univalents split when bivalents underwent reductional division in anaphase I.”
e1“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
Few laggard chromosomes or chromatids occurred in anaphase I.
Verbatim
e0“There were few laggard chromosomes or chromatids at this phase.”
e1“anaphase I.”
e2“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
F3 individuals had a high, variable chromosome number of 48–58.
Verbatim
e0“number of chromosomes (2n = 48-58) was high and variable”
e1“F (3) individuals”
e2“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
F3 individuals had a high bivalent count of 18–23 per cell.
Verbatim
e0“number of bivalents per cell (18-23) also high in F (3) individuals”
e1“tricepiro (Don Santiago x Don Noé)”
Claim · model-written summary
All examined F8 Don Santiago × Don Noé tricepiros had 42 chromosomes and retained the rye genome.
Verbatim
e0“In all F (8) tricepiros (Don Santiago x Don Noé), F (12) tricepiros (Cumé x Horovitz) and F (12) tricepiros (Cumé x Don Noé), the number of chromosomes (2n = 42) was the same, these retaining the rye genome”
Claim · model-written summary
All examined F12 Cumé × Horovitz tricepiros had 42 chromosomes and retained the rye genome.
Verbatim
e0“In all F (8) tricepiros (Don Santiago x Don Noé), F (12) tricepiros (Cumé x Horovitz) and F (12) tricepiros (Cumé x Don Noé), the number of chromosomes (2n = 42) was the same, these retaining the rye genome”
Claim · model-written summary
All examined F12 Cumé × Don Noé tricepiros had 42 chromosomes and retained the rye genome.
Verbatim
e0“In all F (8) tricepiros (Don Santiago x Don Noé), F (12) tricepiros (Cumé x Horovitz) and F (12) tricepiros (Cumé x Don Noé), the number of chromosomes (2n = 42) was the same, these retaining the rye genome”