Abstract
BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metaboliteproduced by Aspergillus species that contaminates a wide range ofagricultural products. OBJECTIVES: This study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of AFB1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in food and feed industry. METHODS:Following successful optimization, using an indirect competitiveimmunoassay in dot blot format, AFB1-bovine serum albumin(AFB1-BSA) conjugate was contact-printed onto 16 isolated subarrayson multi-pad nitrocellulose coated slides; subsequently usedin competitive binding assays. RESULTS: Using the aforementionedassay, AFB1 was determined from 15 pg/g to 3.04 ng/g workingrange with detection limit (LOD) of 1 pg/g. To evaluate assayperformance in real food matrices, the authors spiked wheatsamples with different concentration of AFB1. After extraction,working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g wasdetermined. Good recoveries (94±9%) were obtained, demonstratingaccurate detection of AFB1 concentrations in wheat samples.Assay procedure completed in 3 hours. CONCLUSIONS: The resultsindicated that the proposed developed assay in microarray formatcould be used for rapid and sensitive detection of AFB1in wheatsamples.
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Claims 6
Claim · model-written summary
The AFB1 microarray assay has a detection limit (LOD) of 1 pg/g.
Not reportedTaxon
Verbatim
e0“with detection limit (LOD) of 1 pg/g”
Claim · model-written summary
The AFB1 microarray assay has a working range of 15 pg/g to 3.04 ng/g.
Not reportedTaxon
Verbatim
e0“AFB1 was determined from 15 pg/g to 3.04 ng/g workingrange”
Claim · model-written summary
In spiked wheat samples the AFB1 assay working range was 0.11-4.15 ng/g.
- Taxon
- wheat
Triticum aestivume1 - Outcome
- working rangese2
- Value
- 0.11-4.15 ng/ge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To evaluate assay performance in real food matrices, the authors spiked wheat samples with different concentration of AFB1”
e2“working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g”
Claim · model-written summary
In spiked wheat samples the AFB1 assay detection limit was 30 pg/g.
- Taxon
- wheatTriticum aestivume1
- Outcome
- detection limite2
- Value
- 30pg/ge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To evaluate assay performance in real food matrices, the authors spiked wheat samples with different concentration of AFB1”
e2“working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g”
Claim · model-written summary
Recoveries of AFB1 from spiked wheat samples were good (94±9%).
- Taxon
- wheatTriticum aestivume1
- Outcome
- recoveriese3
- Value
- 94±9%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“To evaluate assay performance in real food matrices, the authors spiked wheat samples with different concentration of AFB1”
e3“Good recoveries (94±9%) were obtained”
Claim · model-written summary
The microarray AFB1 assay procedure was completed in 3 hours.
Not reportedTaxon
Verbatim
e4“Assay procedure completed in 3 hours”