Abstract
Potato is an important and highly valued crop throughout the Maltese Archipelago. Much of the production is exported to Holland. In January 2005, minor wilts and chlorosis of potato plants were observed in a field at Hal-Farrug, Luqa (Malta). Verticillium dahliae Kleb (1) was isolated on potato dextrose agar (PDA) from vascular tissue excised from the base of the plants. Three different isolates were obtained, all of which were typically distinguished by verticillately shaped conidiophores and the abundant production of microsclerotia on PDA. In May 2005, colonies of these three isolates were cultured in potato dextrose broth (PDB), from which conidial suspensions of each isolate were prepared with sterile distilled water to a concentration of 10 7 ml -1 . For each isolate, 10 7-day-old potato seedlings were inoculated via root immersion in the inoculum suspension and transplanted to 20-cm diameter plastic pots containing a soil/peat mixture (1:1 [v/v]). Seedlings treated in the same way with sterile distilled water were used as a control. All plants were kept under controlled glasshouse conditions (20 ± 3°C) and watered to field capacity as required. Minor chlorosis and wilt of the pair of lower-most leaves was noted 7 days after inoculation. During subsequent weeks, wilt began to appear in the typical half-leaf form, while chlorosis was noted on all organs of the plants, including the principal stem (3). Symptoms were absent on the control plants. Measuring the weight of the new tubers produced by each plant revealed no apparent difference between inoculated and healthy plants; nevertheless, inoculated plants resulted in more tubers with a smaller diameter in respect to those of the uninoculated plants. V. dahliae was never isolated from tubers. Little to no variation in symptom severity was noted among plants inoculated with the three individual isolates. At the end of June, V. dahliae was reisolated on PDA from all inoculated plants, in particular, from vascular tissues originating from principal and lateral stems, crowns, and roots. All attempts to isolate the pathogen from control plants were unsuccessful. Molecular detection of the pathogen by using species-specific primers and real-time Scorpion PCR (2) confirmed the results obtained by the classical isolation method. The low symptom severity observed by the growers in the field, usually mistaken for normal dieback of aged plants, might explain why V. dahliae was never reported before on potatoes in the Maltese Archipelago. References: (1) D. L. Hawksworth and P. W. Talboys. No. 256. Descriptions of Pathogenic Fungi and Bacteria. Commonwealth Mycological Institute (CMI), Kew, Surrey, UK, 1970. (2) F. Nigro et al. Pages 454–461 in: Proc. Convegno Internazionale di Olivicoltura. VI Giornate Scientifiche SOI, Spoleto, 2002. (3) W. R. Stevenson et al., eds. Compendium of Potato Diseases. 2nd ed. The American Phytopathological Society, St Paul, MN, 2001.
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Claims 9
Claim · model-written summary
Potato wilting and chlorosis were observed in a field in Malta.
- Taxon
- potato
Solanum tuberosume0 - Outcome
- minor wilts and chlorosise0
- Setting
- Hal-Farrug, Luqa (Malta)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“minor wilts and chlorosis of potato plants were observed in a field at Hal-Farrug, Luqa (Malta)”
Claim · model-written summary
Verticillium dahliae was isolated from potato vascular tissue.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- vascular tissuee0
- Interactor
- Verticillium dahliae
Verticillium dahliaee0pathogen - Outcome
- Verticillium dahliae Kleb (1) was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae Kleb (1) was isolated on potato dextrose agar (PDA) from vascular tissue excised from the base of the plants”
Claim · model-written summary
Inoculated seedlings developed chlorosis and wilt while controls had no symptoms.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculatione0
- Outcome
- chlorosis and wilte0
- Direction
- was notede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Minor chlorosis and wilt of the pair of lower-most leaves was noted 7 days after inoculation. During subsequent weeks, wilt began to appear in the typical half-leaf form, while chlorosis was noted on all organs of the plants, including the principal stem (3). Symptoms were absent on the control plants.”
t0“Potato”
Claim · model-written summary
New tuber weight showed no apparent difference between inoculated and healthy plants.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculatede0
- Outcome
- weight of the new tuberse0
- Direction
- no apparent differencee0
- Comparator
- healthy plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“weight of the new tubers produced by each plant revealed no apparent difference between inoculated and healthy plants”
t0“Potato”
Claim · model-written summary
Inoculated plants produced more, smaller tubers than uninoculated plants.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculated plantse0
- Outcome
- more tubers with a smaller diametere0
- Direction
- moree0
- Comparator
- uninoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculated plants resulted in more tubers with a smaller diameter in respect to those of the uninoculated plants”
t0“Potato”
Claim · model-written summary
Verticillium dahliae was not isolated from tubers.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- V. dahliae was never isolated from tuberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. dahliae was never isolated from tubers”
t0“Potato”
Claim · model-written summary
Symptom severity varied little among the three isolates.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- symptom severitye0
- Direction
- Little to no variatione0
- Qualifier
- three individual isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Little to no variation in symptom severity was noted among plants inoculated with the three individual isolates”
t0“Potato”
Claim · model-written summary
Verticillium dahliae was reisolated from every inoculated plant and not from controls.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculated plantse0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- V. dahliae was reisolatede0
- Comparator
- control plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. dahliae was reisolated on PDA from all inoculated plants, in particular, from vascular tissues originating from principal and lateral stems, crowns, and roots. All attempts to isolate the pathogen from control plants were unsuccessful.”
t0“Potato”
Claim · model-written summary
Species-specific real-time PCR confirmed classical isolation results.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- Molecular detection of the pathogene0
- Comparator
- classical isolation methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular detection of the pathogen by using species-specific primers and real-time Scorpion PCR (2) confirmed the results obtained by the classical isolation method”
t0“Potato”
The same questions in other papers
inoculation · physiology: pigments · Solanum tuberosum (potato)Positive and negative results
8 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.