Abstract
Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae. Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting (ta)-siRNAs, most of which target mRNAs of defense-related proteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e-derived ta-siRNA-mediated silencing on NBS-LRR-disease-resistance proteins. It is speculated that a miR482-mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection.
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Claims 6
Claim · model-written summary
Potato miR482 variants target NBS-LRR disease resistance proteins.
- Taxon
- potato
Solanum tuberosume0 - Gene
- miR482e0
- Outcome
- target a class of disease-resistance proteinse0
- Qualifier
- variable in sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease-resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs.”
Claim · model-written summary
V. dahliae infection downregulated miR482e expression in potato plantlets.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- infected with V. dahliaee0
- Interactor
- V. dahliae
Verticillium dahliaee0pathogen - Gene
- miR482ee0
- Outcome
- expression level of miR482ee0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated”
Claim · model-written summary
V. dahliae infection upregulated NBS-LRR targets of miR482e.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- infected with V. dahliaee0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- several NBS-LRR targets of miR482ee0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS-LRR targets of miR482e were upregulated.”
Claim · model-written summary
Overexpressing miR482e increased potato sensitivity to V. dahliae infection.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- overexpressing miR482ee0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- miR482ee0
- Outcome
- hypersensitivity to V. dahliae infectione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection.”
Claim · model-written summary
miR482e targets NBS-LRR disease-resistance protein mRNAs.
Not reportedTaxon
Verbatim
e0“we validated that miR482e targets mRNAs of NBS-LRR disease-resistance proteins”
Claim · model-written summary
miR482e triggers ta-siRNAs that mostly target defense-related protein mRNAs.
- Gene
- miR482ee0
- Outcome
- production of trans-acting (ta)-siRNAse0
- Qualifier
- most of which target mRNAs of defense-related proteinse0
Not reportedTaxon
Verbatim
e0“miR482e targets mRNAs of NBS-LRR disease-resistance proteins and triggers the production of trans-acting (ta)-siRNAs, most of which target mRNAs of defense-related proteins.”
The same questions in other papers
infection · molecular: expression · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 17 in the opposite direction
- positive
- positive
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.