Abstract
Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean expressed sequence tags (ESTs) generated from more than 50 cDNA libraries were evaluated. These ESTs coalesced into 16928 contigs and 17336 singletons. On average, each contig was composed of 6 ESTs and spanned 788 bases. The average sequence length submitted to dbEST was 414 bases. Using only those libraries generating more than 800 ESTs each and only those contigs with 10 or more ESTs each, correlated patterns of gene expression among libraries and genes were discerned. Two-dimensional qualitative representations of contig and library similarities were generated based on expression profiles. Genes with similar expression patterns and, potentially, similar functions were identified. These studies provide a rich source of publicly available gene sequences as well as valuable insight into the structure, function, and evolution of a model crop legume genome.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Soybean ESTs assembled into 16928 contigs and 17336 singletons.
- Taxon
- soybean
Glycine maxt0 - Outcome
- contigse0
- Value
- 16928 contigse0
- Qualifier
- 17336 singletonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“These ESTs coalesced into 16928 contigs and 17336 singletons.”
Claim · model-written summary
Soybean contigs averaged six ESTs and 788 bases.
- Taxon
- soybeanGlycine maxt0
- Outcome
- contige0
- Value
- 6 ESTse0
- Qualifier
- 788 basese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“On average, each contig was composed of 6 ESTs and spanned 788 bases.”
Claim · model-written summary
The average soybean EST sequence submitted to dbEST was 414 bases.
- Taxon
- soybeanGlycine maxt0
- Outcome
- average sequence lengthe0
- Value
- 414 basese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“The average sequence length submitted to dbEST was 414 bases.”
Claim · model-written summary
Correlated gene-expression patterns among soybean libraries and genes were discerned.
- Taxon
- soybeanGlycine maxt0
- Outcome
- correlated patterns of gene expression among libraries and genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“correlated patterns of gene expression among libraries and genes were discerned”
Claim · model-written summary
Two-dimensional representations of soybean contig and library similarities were generated from expression profiles.
- Taxon
- soybeanGlycine maxt0
- Outcome
- representations of contig and library similaritiese0
- Value
- Two-dimensionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Two-dimensional qualitative representations of contig and library similarities were generated based on expression profiles.”
Claim · model-written summary
Genes with similar soybean expression patterns and potentially similar functions were identified.
- Taxon
- soybeanGlycine maxt0
- Outcome
- Genes with similar expression patterns and, potentially, similar functionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Genes with similar expression patterns and, potentially, similar functions were identified.”