Abstract
Plant cells, tissues and organ cultures initiated from twenty-one plants were examined for the presence of bacterial contamination. The plants were mostly medicinal plants from St. Katherine, Sinai, Egypt. The explants were mainly derived from sterile seedling parts. In vitro cultures initiated from Sinai’s medicinal plants were not prone to bacterial contamination. Occasional occurrence of Methylobacterium mesophilicum (PPFM) on plates cultured with Datura stramonium was observed from slightly old cultures. Contamination with Mycobacterium scrofulaceum infrequently occured from explants derived from mature plants grown under green house conditions. Antibiotic sensitivity of Methylobacterium mesophilicum and Mycobacterium scrofulaceum were investigated against sixteen different antibiotics using the disc diffusion method. All antibiotic tested were inhibitory to Mycobacterium scrofulaceum, while only seven of the tested antibiotics inhibited the growth of Methylobacterium mesophilicum. Though the antibiotic sensitivity of those two bacteria against various antibiotics was variant, both bacteria showed the widest inhibition zone against Refampacin and Imipenem.
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Claims 6
Claim · model-written summary
In vitro cultures of Sinai medicinal plants were not prone to bacterial contamination.
Verbatim
e0“The plants were mostly medicinal plants from St. Katherine, Sinai, Egypt”
e1“were not prone to bacterial contamination”
Claim · model-written summary
Methylobacterium mesophilicum occasionally occurred on Datura stramonium cultures from slightly old cultures.
- Taxon
- Datura stramonium
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Datura stramoniume2 - Interactor
- Methylobacterium mesophilicumMethylobacterium mesophilicume2other
- Outcome
- Occasional occurrencee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e2“Occasional occurrence of Methylobacterium mesophilicum (PPFM) on plates cultured with Datura stramonium was observed from slightly old cultures”
Claim · model-written summary
Mycobacterium scrofulaceum infrequently contaminated explants from mature plants grown under greenhouse conditions.
Verbatim
e3“Contamination with Mycobacterium scrofulaceum infrequently occured from explants derived from mature plants grown under green house conditions”
Claim · model-written summary
All antibiotics tested inhibited Mycobacterium scrofulaceum.
- Taxon
- medicinal plantse0
- Treatment
- All antibiotic testede4
- Interactor
- Mycobacterium scrofulaceumMycobacterium scrofulaceume4other
- Outcome
- inhibitorye4
Not reportedDirection
Verbatim
e0“The plants were mostly medicinal plants from St. Katherine, Sinai, Egypt”
e4“All antibiotic tested were inhibitory to Mycobacterium scrofulaceum”
Claim · model-written summary
Only seven of the tested antibiotics inhibited the growth of Methylobacterium mesophilicum.
Verbatim
e0“The plants were mostly medicinal plants from St. Katherine, Sinai, Egypt”
e5“only seven of the tested antibiotics inhibited the growth of Methylobacterium mesophilicum”
Claim · model-written summary
Both bacteria showed the widest inhibition zone against Refampacin and Imipenem.
- Taxon
- medicinal plantse0
- Treatment
- Refampacin and Imipeneme6
- Interactor
- Methylobacterium mesophilicumMethylobacterium mesophilicume7other
- Second interactor
- Mycobacterium scrofulaceumMycobacterium scrofulaceume7other
- Outcome
- inhibition zonee6
- Direction
- wideste6
Verbatim
e0“The plants were mostly medicinal plants from St. Katherine, Sinai, Egypt”
e6“both bacteria showed the widest inhibition zone against Refampacin and Imipenem”
e7“Antibiotic sensitivity of Methylobacterium mesophilicum and Mycobacterium scrofulaceum were investigated against sixteen different antibiotics”