Paper · 1965 · Journal of Experimental Botany

A Vapour-pressure Instrument for the Measurement of Leaf and Soil Water Potential

A. E. S. MACKLON; P. E. WEATHERLEY

6
claims
6
verbatim quotes
22
citations
2
research areas
—
Not open access

Abstract

A previously described vapour-pressure osmometer has been adapted for the measurement of depression of water potential (DPD) in samples of leaf tissue and soil. A pile of about 30 leaf annuli are contained in an air-tight metal capsule through the lid of which passes a pipette. A drop of water hangs from the tip of the pipette into the central cavity of the pile of leaf annuli. The drop is drawn bath into the pipette from time to time and the rate of evaporation from it measured directly. The water-potential depression is linearly related to the rate of evaporation. The instrument is calibrated empincally. A period of a hours is necessary to attain vapour equilibnum before readings are taken The readings then occupy less than 30 minutes. For the calibration data, using filter paper moistened with NaCl solution, the 5 percent inverse tolerance limits for single readings of water-potential depression were ±0.26 atm. With leaf material the errors appear to be about the same, although release of cell sap at the cut edges of the disks may cause an overestimation by as much as 0.5 atm. with turgid leaf material. Lining the capsule with leaf strips gave erroneous results. Using a suitably modified capsule the instrument can be used for soil-water potential measurement. Equilibration takes only 30 minutes so that a reading can be completed within an hour.

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Claims 6

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Water-potential depression was linearly related to drop evaporation rate.

Outcome
water-potential depressione0

Not reportedTaxonComparatorDirectionValue

Verbatim

e0

“The water-potential depression is linearly related to the rate of evaporation”

✓ located verbatim in the source · abstract, characters 494–571 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Single-reading tolerance limits were ±0.26 atm in filter-paper calibration.

Outcome
inverse tolerance limits for single readings of water-potential depressione0
Value
±0.26 atme0
Qualifier
5 percente0

Not reportedTaxon

Verbatim

e0

“the 5 percent inverse tolerance limits for single readings of water-potential depression were ±0.26 atm”

✓ located verbatim in the source · abstract, characters 823–926 · show in text ↑
Method / resourceauthor interpretationoriginal resultmeasured

Claim · model-written summary

Leaf-material errors appeared similar to calibration errors.

Outcome
errorse0
Direction
about the samee0
Value
0.5 atme0
Comparator
about the samee0

Not reportedTaxon

Verbatim

e0

“With leaf material the errors appear to be about the same, although release of cell sap at the cut edges of the disks may cause an overestimation by as much as 0.5 atm”

✓ located verbatim in the source · abstract, characters 928–1095 · show in text ↑
Method / resourceauthor interpretationoriginal resultmeasured

Claim · model-written summary

Sap release at cut leaf-disk edges may overestimate water-potential depression by as much as 0.5 atm.

Organ / stage
cell sape0
Outcome
overestimatione0
Direction
overestimatione0
Value
0.5 atme0

Not reportedTaxon

Verbatim

e0

“release of cell sap at the cut edges of the disks may cause an overestimation by as much as 0.5 atm”

✓ located verbatim in the source · abstract, characters 996–1095 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Leaf-strip lining yielded erroneous results.

Treatment
Lining the capsule with leaf stripse0
Outcome
erroneous resultse0

Not reportedTaxonComparatorValue

Verbatim

e0

“Lining the capsule with leaf strips gave erroneous results”

✓ located verbatim in the source · abstract, characters 1124–1182 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A modified capsule allowed soil-water potential readings within an hour after 30 minutes of equilibration.

Outcome
soil-water potential measuremente0
Value
30 minutese0
Qualifier
within an houre0

Not reportedTaxon

Verbatim

e0

“Using a suitably modified capsule the instrument can be used for soil-water potential measurement. Equilibration takes only 30 minutes so that a reading can be completed within an hour”

✓ located verbatim in the source · abstract, characters 1184–1368 · show in text ↑