Abstract
Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits practicability in the plant acquisition and drying process, especially when the collection is performed in situ, and also because its simplicity makes possible the cold storage of the dry, ground samples for future DNA extractions.
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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
All three modified CTAB DNA extraction methods gave satisfactory DNA quantity, purity and microsatellite band patterns in sweetpotato landraces.
- Taxon
- sweetpotato
_Flower.jpg?width=60)
Ipomoea batatast0 - Outcome
- satisfactory resultse0
- Comparator
- three modified methodologiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“sweetpotato”
e0“All methodologies yielded satisfactory results”
e1“three modified methodologies for DNA extraction”
Claim · model-written summary
The method using leaf tissue macerated in liquid nitrogen was judged the most adequate of the three because of its simplicity and lower cost.
- Taxon
- sweetpotatoIpomoea batatast0
- Outcome
- more adequatee0
- Qualifier
- simplicity and lower coste0
- Comparator
- three modified methodologiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweetpotato”
e0“the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost”
e1“three modified methodologies for DNA extraction”
Claim · model-written summary
The method using dry leaf tissue was considered more advantageous for its practicability in plant acquisition and drying and because dry ground samples can be cold-stored.
- Taxon
- sweetpotatoIpomoea batatast0
- Outcome
- more advantageouse0
- Comparator
- three modified methodologiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweetpotato”
e0“the method based in dry leaf tissue was considered more advantageous”
e1“three modified methodologies for DNA extraction”