Abstract
The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a member of the bipartite geminiviruses infecting dicotyledonous plants, has been compared in host and non-host cells. A derivative of the ACMV DNA A was transfected into tobacco and maize protoplasts. Although ACMV is not able to infect maize, replication of the DNA A in maize protoplasts was observed to occur. The efficiency of replication was 10 to 20% of that seen in tobacco protoplasts. In both plant systems, replication was detected after the onset of cell division. ACMV replication in maize cells was compared to that of wheat dwarf virus and found to be 10 to 20% of that observed with the monocotyledon-specific virus. Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco.
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Claims 6
Claim · model-written summary
ACMV DNA A replicated in maize protoplasts.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“replication of the DNA A in maize protoplasts was observed to occur”
t0“African cassava mosaic virus (ACMV)”
Claim · model-written summary
ACMV DNA A replication in maize protoplasts was 10 to 20% of replication in tobacco protoplasts.
- Interactor
- African cassava mosaic virusBegomovirus manihotist0pathogen
- Outcome
- efficiency of replicatione0
- Direction
- lowerinferrede0
- Value
- 10 to 20%e0
- Comparator
- tobacco protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The efficiency of replication was 10 to 20% of that seen in tobacco protoplasts”
t0“African cassava mosaic virus in a heterologous system”
e1“replication of the DNA A in maize protoplasts was observed to occur”
Claim · model-written summary
Replication was detected after cell division began in both plant systems.
- Interactor
- African cassava mosaic virusBegomovirus manihotist0pathogen
- Outcome
- replication was detected after the onset of cell divisione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In both plant systems, replication was detected after the onset of cell division”
t0“African cassava mosaic virus in a heterologous system”
e1“replication of the DNA A in maize protoplasts was observed to occur”
Claim · model-written summary
ACMV replication in maize cells was 10 to 20% of wheat dwarf virus replication.
- Interactor
- ACMVe0pathogen
- Second interactor
- wheat dwarf virusWheat dwarf viruse0pathogen
- Outcome
- ACMV replicatione0
- Direction
- lowerinferrede0
- Value
- 10 to 20%e0
- Comparator
- wheat dwarf viruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACMV replication in maize cells was compared to that of wheat dwarf virus and found to be 10 to 20% of that observed with the monocotyledon-specific virus”
Claim · model-written summary
Insertion of 1165 bp of non-viral DNA prevented ACMV DNA A replication in maize.
- Treatment
- Insertion of 1165 bp of non-viral DNAe0
- Interactor
- ACMVe0pathogen
- Outcome
- replication in maizee0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco”
Claim · model-written summary
Insertion of 1165 bp of non-viral DNA did not prevent ACMV DNA A replication in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Insertion of 1165 bp of non-viral DNAe0
- Interactor
- ACMVe0pathogen
- Outcome
- replicatione0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco”