Abstract
BACKGROUND: The recovery of high performing transgenic lines in clonal crops is limited by the occurrence of somaclonal variation during the tissue culture phase of transformation. This is usually circumvented by developing large populations of transgenic lines, each derived from the first shoot to regenerate from each transformation event. This study investigates a new strategy of assessing multiple shoots independently regenerated from different transformed cell colonies of potato (Solanum tuberosum L.). RESULTS: A modified cry9Aa2 gene, under the transcriptional control of the CaMV 35S promoter, was transformed into four potato cultivars using Agrobacterium-mediated gene transfer using a nptII gene conferring kanamycin resistance as a selectable marker gene. Following gene transfer, 291 transgenic lines were grown in greenhouse experiments to assess somaclonal variation and resistance to potato tuber moth (PTM), Phthorimaea operculella (Zeller). Independently regenerated lines were recovered from many transformed cell colonies and Southern analysis confirmed whether they were derived from the same transformed cell. Multiple lines regenerated from the same transformed cell exhibited a similar response to PTM, but frequently exhibited a markedly different spectrum of somaclonal variation. CONCLUSIONS: A new strategy for the genetic improvement of clonal crops involves the regeneration and evaluation of multiple shoots from each transformation event to facilitate the recovery of phenotypically normal transgenic lines. Most importantly, regenerated lines exhibiting the phenotypic appearance most similar to the parental cultivar are not necessarily derived from the first shoot regenerated from a transformed cell colony, but can frequently be a later regeneration event.
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Claims 4
Claim · model-written summary
Lines regenerated from the same transformed cell showed a similar response to potato tuber moth
- Taxon
- potato
Solanum tuberosume0 - Interactor
- Phthorimaea operculella
Phthorimaea operculellae2pest - Outcome
- response to PTMe1
- Qualifier
- from the same transformed celle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“multiple shoots independently regenerated from different transformed cell colonies of potato (Solanum tuberosum L.)”
e1“Multiple lines regenerated from the same transformed cell exhibited a similar response to PTM”
e2“resistance to potato tuber moth (PTM), Phthorimaea operculella (Zeller)”
Claim · model-written summary
Lines regenerated from the same transformed cell frequently showed a markedly different spectrum of somaclonal variation
- Taxon
- potatoSolanum tuberosume0
- Outcome
- spectrum of somaclonal variatione3
- Qualifier
- markedly differente3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“multiple shoots independently regenerated from different transformed cell colonies of potato (Solanum tuberosum L.)”
e3“but frequently exhibited a markedly different spectrum of somaclonal variation”
Claim · model-written summary
Regenerated lines most similar in phenotype to the parental cultivar are not necessarily from the first shoot and can frequently be later regenerations
- Taxon
- potatoSolanum tuberosume0
- Outcome
- phenotypic appearance most similar to the parental cultivare4
- Qualifier
- not necessarily derived from the first shoote4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“multiple shoots independently regenerated from different transformed cell colonies of potato (Solanum tuberosum L.)”
e4“Most importantly, regenerated lines exhibiting the phenotypic appearance most similar to the parental cultivar are not necessarily derived from the first shoot regenerated from a transformed cell colony, but can frequently be a later regeneration event”
Claim · model-written summary
Regenerating and evaluating multiple shoots from each transformation event is recommended for the genetic improvement of clonal crops
- Taxon
- potatoSolanum tuberosume0
- Treatment
- regeneration and evaluation of multiple shootse5
- Outcome
- recovery of phenotypically normal transgenic linese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“multiple shoots independently regenerated from different transformed cell colonies of potato (Solanum tuberosum L.)”
e5“A new strategy for the genetic improvement of clonal crops involves the regeneration and evaluation of multiple shoots from each transformation event to facilitate the recovery of phenotypically normal transgenic lines”