Abstract
With the aim of introducing anther culture to Hemerocallis spp., effects of basal mediums, growth regulators and addition agents on callus formation and plant regeneration were studied. Highest callus formation rate and plant differentiation of both H. dumortieri and H. fulva var. kwanso were obtained when the medium was inoculated to the flower bud size of 0.51.0 cm in length. When B5 medium was used as basal medium, the callus and plantlet were hardly generated. But the callus formation and plant differentiation were observed in all treatments with MS medium. Among the treatments of growth regulators and varying degrees of concentration, highest callus formation and plantlet regeneration were observed in NAA 2.0＋BA 2.0 mg․ combination for H. dumortieri, and kinetin 1.0＋2.4-D 1.0 mg․ combination for H. fulva var. kwanso, respectively. Among temperature treatments, the callus formation was high for all species when treated at 35 for one day, but the plantlet regeneration was incomplete under the same condition. However, the plantlet regeneration was observed in all species when treated at 4 for four days. Addition of glutamine resulted in high callus formation in all the species, whereas charcoal addition resulted in lower callus formation in H. fulva var. kwanso than control. No response was observed in H. dumortieri.
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Claims 12
Claim · model-written summary
Flower buds of 0.51.0 cm gave the highest callus formation and differentiation in H. dumortieri.
- Taxon
- H. dumortieri
Hemerocallis dumortierie0 - Treatment
- flower bud size of 0.51.0 cme0
- Outcome
- callus formation rate and plant differentiatione0
- Direction
- Higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest callus formation rate and plant differentiation of both H. dumortieri and H. fulva var. kwanso were obtained when the medium was inoculated to the flower bud size of 0.51.0 cm in length”
Claim · model-written summary
Flower buds of 0.51.0 cm gave the highest callus formation and differentiation in H. fulva var. kwanso.
- Taxon
- H. fulva var. kwanso
Hemerocallis fulva var. kwansoe0 - Treatment
- flower bud size of 0.51.0 cme0
- Outcome
- callus formation rate and plant differentiatione0
- Direction
- Higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest callus formation rate and plant differentiation of both H. dumortieri and H. fulva var. kwanso were obtained when the medium was inoculated to the flower bud size of 0.51.0 cm in length”
Claim · model-written summary
B5 medium hardly generated callus or plantlets.
- Taxon
- Hemerocallis spp.
_v2.jpg?width=60)
Hemerocallist0 - Treatment
- B5 mediume0
- Outcome
- callus and plantlet were hardly generatede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_v2.jpg?width=500)
Verbatim
e0“When B5 medium was used as basal medium, the callus and plantlet were hardly generated.”
t0“Hemerocallis spp.”
Claim · model-written summary
MS medium produced callus formation and plant differentiation in all treatments.
- Taxon
- Hemerocallis spp.Hemerocallist0
- Treatment
- MS mediume0
- Outcome
- callus formation and plant differentiation were observede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the callus formation and plant differentiation were observed in all treatments with MS medium”
t0“Hemerocallis spp.”
Claim · model-written summary
NAA 2.0＋BA 2.0 mg․ gave the highest callus formation and regeneration in H. dumortieri.
- Taxon
- H. dumortieriHemerocallis dumortierie0
- Treatment
- NAA 2.0＋BA 2.0 mg․e0
- Outcome
- callus formation and plantlet regeneratione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest callus formation and plantlet regeneration were observed in NAA 2.0＋BA 2.0 mg․ combination for H. dumortieri”
Claim · model-written summary
Kinetin 1.0＋2.4-D 1.0 mg․ gave the highest callus formation and regeneration in H. fulva var. kwanso.
- Taxon
- H. fulva var. kwansoHemerocallis fulva var. kwansoe0
- Treatment
- kinetin 1.0＋2.4-D 1.0 mg․e0
- Outcome
- callus formation and plantlet regeneratione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest callus formation and plantlet regeneration were observed in NAA 2.0＋BA 2.0 mg․ combination for H. dumortieri, and kinetin 1.0＋2.4-D 1.0 mg․ combination for H. fulva var. kwanso”
Claim · model-written summary
Treatment at 35 for one day gave high callus formation.
- Taxon
- Hemerocallis spp.Hemerocallist0
- Treatment
- 35 for one daye0
- Outcome
- callus formatione0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the callus formation was high for all species when treated at 35 for one day”
t0“Hemerocallis spp.”
Claim · model-written summary
Plantlet regeneration was incomplete after treatment at 35 for one day.
- Taxon
- Hemerocallis spp.Hemerocallist0
- Treatment
- 35 for one daye0
- Outcome
- plantlet regeneration was incompletee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when treated at 35 for one day, but the plantlet regeneration was incomplete under the same condition”
t0“Hemerocallis spp.”
Claim · model-written summary
Treatment at 4 for four days allowed plantlet regeneration in all species.
- Taxon
- Hemerocallis spp.Hemerocallist0
- Treatment
- 4 for four dayse0
- Outcome
- plantlet regeneration was observede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plantlet regeneration was observed in all species when treated at 4 for four days”
t0“Hemerocallis spp.”
Claim · model-written summary
Glutamine addition gave high callus formation.
- Taxon
- Hemerocallis spp.Hemerocallist0
- Treatment
- Addition of glutaminee0
- Outcome
- callus formatione0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of glutamine resulted in high callus formation in all the species”
t0“Hemerocallis spp.”
Claim · model-written summary
Charcoal addition reduced callus formation in H. fulva var. kwanso.
- Taxon
- H. fulva var. kwansoHemerocallis fulva var. kwansoe0
- Treatment
- charcoal additione0
- Outcome
- callus formatione0
- Direction
- lowere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“charcoal addition resulted in lower callus formation in H. fulva var. kwanso than control”
Claim · model-written summary
H. dumortieri showed no response to charcoal addition.
- Taxon
- H. dumortieriHemerocallis dumortierie0
- Treatment
- charcoal additione1
- Outcome
- No responsee0
- Comparator
- controle1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No response was observed in H. dumortieri.”
e1“charcoal addition resulted in lower callus formation in H. fulva var. kwanso than control”
The same questions in other papers
in vitro · growth · HemerocallisResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.