Paper · 2011 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Genetic diversity in 14 tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets by RAPD-PCR technique

CN Ezekiel; CC Nwangburuka; OA Ajibade; et al.

8
claims
16
verbatim quotes
17
citations
1
research areas
✓
Open access

Abstract

A total of 226 tomato (Lycopersicon esculentum Mill.) fruits sold in markets within three states in Southwestern Nigeria were purchased and differentiated into 14 varietal groups based on morphological parameters. The genomic DNA from the young apical leaves of the seeded plants were extracted and analyzed by the randomly amplified polymorphic DNA-polymerase chain reaction (RAPDPCR) technique. Seventy-four (74) amplified products were scored with 62.2% of them revealing polymorphism. The OPB-18 primer recorded the highest polymorphism (83.3%) and OPU-14 the least (44.4%). The dendrogram revealed 3 clusters. The genotypic relationships between varieties within clusters ranged from 2 in clusters 1 and 3 to 10 in cluster 2. Cluster 2 revealed 4 subgroups with two pairs of varieties (var. c and i and l and m) showing 100% similarities. The local names, place of cultivation and phenotypic characters expressed by the varieties were not suitable for varietal differentiation since phenotypically different fruits showed genotypic relatedness. The impression that tomato varieties from a particular geographical zone are genetically similar was therefore nullified.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Of 74 amplified RAPD products scored in the tomato varieties, 62.2% were polymorphic.

Outcome
polymorphisme0
Value
62.2%e0
Setting
Nigerian marketst0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“Seventy-four (74) amplified products were scored with 62.2% of them revealing polymorphism”

✓ located verbatim in the source · abstract, characters 397–487 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The OPB-18 primer gave the highest polymorphism, 83.3%.

Outcome
polymorphisme0
Value
83.3%e0
Qualifier
OPB-18 primere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“The OPB-18 primer recorded the highest polymorphism (83.3%) and OPU-14 the least (44.4%)”

✓ located verbatim in the source · abstract, characters 489–577 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The OPU-14 primer gave the lowest polymorphism, 44.4%.

Outcome
polymorphisme0
Value
44.4%e0
Qualifier
OPU-14e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“The OPB-18 primer recorded the highest polymorphism (83.3%) and OPU-14 the least (44.4%)”

✓ located verbatim in the source · abstract, characters 489–577 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The dendrogram of the tomato varieties revealed three clusters.

Outcome
clusterse0
Value
3 clusterse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“The dendrogram revealed 3 clusters”

✓ located verbatim in the source · abstract, characters 579–613 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cluster 2 split into four subgroups, with two pairs of varieties showing 100% similarity.

Outcome
similaritiese0
Value
100%e0
Qualifier
two pairs of varietiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“Cluster 2 revealed 4 subgroups with two pairs of varieties (var. c and i and l and m) showing 100% similarities”

✓ located verbatim in the source · abstract, characters 731–842 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Genotypic relationships between varieties within clusters ranged from 2 in clusters 1 and 3 to 10 in cluster 2.

Outcome
genotypic relationships between varieties within clusterse0
Value
ranged from 2 in clusters 1 and 3 to 10 in cluster 2e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“The genotypic relationships between varieties within clusters ranged from 2 in clusters 1 and 3 to 10 in cluster 2”

✓ located verbatim in the source · abstract, characters 615–729 · show in text ↑
Observationauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Local names, place of cultivation and phenotypic characters were not suitable for differentiating the varieties, since phenotypically different fruits were genotypically related.

Outcome
varietal differentiatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“The local names, place of cultivation and phenotypic characters expressed by the varieties were not suitable for varietal differentiation”

✓ located verbatim in the source · abstract, characters 844–981 · show in text ↑
Observationauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

The idea that tomato varieties from one geographical zone are genetically similar was not supported.

Outcome
genetically similare0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets”

✓ located verbatim in the source · title, characters 24–92 · show in text ↑
e0

“The impression that tomato varieties from a particular geographical zone are genetically similar was therefore nullified”

✓ located verbatim in the source · abstract, characters 1050–1170 · show in text ↑