Abstract
Recombinants of PVY Strains Predominate Among Isolates from Potato Crop in Poland 282 Potato virus Y (PVY) isolates collected from potato crops in northern and central Poland from 1995 to 2009 were characterized by serological and biological assays. From these, 112 isolates collected from 2006 to 2009 were additionally analyzed by one-step triplex Reverse Transcription-Polymeraze Chain Reaction (RT-PCR). Recombinants of PVY strains predominate among the isolates tested. Using one-step triplex RT-PCR most PVY N-Wi isolates were classified as subgroup PVY N Wi-P and most PVY N and/or PVY NTN isolates as the recombinant PVY NTN strain. A recombinant PVY NTN isolate (12/94) and two additional PVY N and/or PVY NTN isolates were not detected by one-step triplex RT-PCR. Twelve isolates were identified as the PVY O strain but PVY N , non-recombinant PVY NTN and PVY C strains were not found. Serological and biological assays of 144 isolates of PVY N-Wi strain showed that 100 isolates were the expected PVY O serotype with vein necrosis (VN) symptoms on tobacco. However, 10 isolates of the PVY N Wi-P subgroup exhibited vein clearing (VCl) on tobacco and 2 isolates of the PVY N N242 subgroup unexpectedly exhibited as the PVY N serotype. All the isolates of PVY N-Wi strain induced severe local lesions (LL) on Chenopodium amaranticolor . Out of 126 isolates of PVY NTN strain tested, 76 were typical PVY N serotype with VN on tobacco, but their reactions on C. amaranticolor were different: 13 isolates did not show symptoms, 23 isolates induced weak, and 40 isolates induced severe LL. The remaining isolates of PVY N-Wi , PVY N and/or PVY NTN or PVY O were serologically PVY N and PVY O positive or exhibited unpredictable serological and biological reactions.
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Claims 14
Claim · model-written summary
Recombinant PVY strains predominated among potato crop isolates.
- Taxon
- Potato
Solanum tuberosumt0 - Interactor
- PVY
Potato virus Ye0pathogen - Outcome
- Recombinants of PVY strains predominatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recombinants of PVY strains predominate among the isolates tested”
t0“Potato Crop”
Claim · model-written summary
Most PVY N-Wi isolates were classified in the PVY N Wi-P subgroup.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- classified as subgroup PVY N Wi-Pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Crop”
e0“most PVY N-Wi isolates were classified as subgroup PVY N Wi-P”
Claim · model-written summary
Most PVY N and/or PVY NTN isolates were classified as recombinant PVY NTN.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- recombinant PVY NTN straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Crop”
e0“most PVY N and/or PVY NTN isolates as the recombinant PVY NTN strain”
Claim · model-written summary
One recombinant and two additional PVY isolates escaped triplex RT-PCR detection.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- not detected by one-step triplex RT-PCRe0
- Value
- two additionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Crop”
e0“A recombinant PVY NTN isolate (12/94) and two additional PVY N and/or PVY NTN isolates were not detected by one-step triplex RT-PCR”
Claim · model-written summary
Twelve isolates were PVY O, while PVY N, non-recombinant PVY NTN and PVY C were absent.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- PVY O straine0
- Value
- Twelve isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Crop”
e0“Twelve isolates were identified as the PVY O strain but PVY N , non-recombinant PVY NTN and PVY C strains were not found”
Claim · model-written summary
Among PVY N-Wi isolates, 100 had PVY O serotype with tobacco vein necrosis.
- Taxon
- tobacco
Nicotiana tabacume0 - Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- vein necrosis (VN) symptomse0
- Value
- 100 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“100 isolates were the expected PVY O serotype with vein necrosis (VN) symptoms on tobacco”
Claim · model-written summary
Ten PVY N Wi-P isolates caused vein clearing on tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- vein clearing (VCl)e0
- Value
- 10 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10 isolates of the PVY N Wi-P subgroup exhibited vein clearing (VCl) on tobacco”
Claim · model-written summary
Two PVY N N242 isolates unexpectedly showed the PVY N serotype.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- PVY N serotypee0
- Value
- 2 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Crop”
e0“2 isolates of the PVY N N242 subgroup unexpectedly exhibited as the PVY N serotype”
Claim · model-written summary
All PVY N-Wi isolates caused severe local lesions on Chenopodium amaranticolor.
- Taxon
- Chenopodium amaranticolor
Chenopodium amaranticolore0 - Interactor
- PVY N-Wie0pathogen
- Outcome
- severe local lesions (LL)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the isolates of PVY N-Wi strain induced severe local lesions (LL) on Chenopodium amaranticolor”
Claim · model-written summary
Of PVY NTN isolates, 76 showed PVY N serotype and vein necrosis on tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- VNe0
- Value
- 76e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“76 were typical PVY N serotype with VN on tobacco”
Claim · model-written summary
Thirteen PVY NTN isolates caused no symptoms on C. amaranticolor.
- Taxon
- C. amaranticolorChenopodium amaranticolore1
- Interactor
- PVY NTNe2pathogen
- Outcome
- did not show symptomse0
- Value
- 13 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“13 isolates did not show symptoms, 23 isolates induced weak, and 40 isolates induced severe LL”
e1“reactions on C. amaranticolor were different”
e2“Out of 126 isolates of PVY NTN strain tested”
Claim · model-written summary
Twenty-three PVY NTN isolates caused weak lesions on C. amaranticolor.
- Taxon
- C. amaranticolorChenopodium amaranticolore1
- Interactor
- PVY NTNe2pathogen
- Outcome
- weake0
- Value
- 23 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“23 isolates induced weak, and 40 isolates induced severe LL”
e1“reactions on C. amaranticolor were different”
e2“Out of 126 isolates of PVY NTN strain tested”
Claim · model-written summary
Forty PVY NTN isolates caused severe lesions on C. amaranticolor.
- Taxon
- C. amaranticolorChenopodium amaranticolore1
- Interactor
- PVY NTNe2pathogen
- Outcome
- severe LLe0
- Value
- 40 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“40 isolates induced severe LL”
e1“reactions on C. amaranticolor were different”
e2“Out of 126 isolates of PVY NTN strain tested”
Claim · model-written summary
Remaining PVY isolates had dual serological positivity or unpredictable assay reactions.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- PVYPotato virus Ye0pathogen
- Outcome
- unpredictable serological and biological reactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato Crop”
e0“The remaining isolates of PVY N-Wi , PVY N and/or PVY NTN or PVY O were serologically PVY N and PVY O positive or exhibited unpredictable serological and biological reactions”