Paper · 2010 · TSpace

Genetic diversity of breeding popcorn lines determined by SSR markers

Ana Paula Ribeiro Trindade; Ronald José Barth Pinto; Antônio Teixeira do Amaral Júnior; et al.

8
claims
15
verbatim quotes
17
citations
2
research areas
✓
Open access

Abstract

Information about genetic dissimilarity is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations. Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers. Of a total of 51 evaluated polymorphic primers, 15 were used for polymerase chain reaction (PCR) amplification. The genetic distance was estimated by Rogers' modified distance. The different popcorn breeding programs in Brazil are possibly using highly similar base-populations. The genetic similarity of lines P1-3 and P8-1 was lowest, while P3-3 and P8-2 were genetically more similar. The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was reliable to discriminate the genotypes in five groups. The clusters were consistent with the estimates of genetic identity. There was a moderate coincidence degree between the groups and genealogy of lines. Higher levels of heterozygosity are expected from crosses between the group containing lines P3-3 and P7-3 with that of P1-3 and P7-4. Crosses between lines P1-3 and P8-1 are also promising.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Genetic similarity was lowest between popcorn lines P1-3 and P8-1.

Taxon
popcorne0
Material
P1-3 and P8-1e1
Outcome
genetic similaritye1
Direction
loweste1

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e1

“The genetic similarity of lines P1-3 and P8-1 was lowest”

✓ located verbatim in the source · abstract, characters 548–604 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Popcorn lines P3-3 and P8-2 were genetically more similar.

Taxon
popcorne0
Material
P3-3 and P8-2e2
Outcome
genetically more similare2

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e2

“while P3-3 and P8-2 were genetically more similar”

✓ located verbatim in the source · abstract, characters 606–655 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

UPGMA was reliable for discriminating the popcorn genotypes into five groups.

Taxon
popcorne0
Outcome
discriminate the genotypese3
Value
five groupse3

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e3

“The cophenetic correlation showed that the Unweighted Pair-Group Method Using Arithmetic Averages (UPGMA) was reliable to discriminate the genotypes in five groups”

✓ located verbatim in the source · abstract, characters 657–820 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The groups and the genealogy of popcorn lines showed a moderate coincidence.

Taxon
popcorne0
Outcome
coincidence degree between the groups and genealogy of linese4
Qualifier
moderatee4

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e4

“There was a moderate coincidence degree between the groups and genealogy of lines”

✓ located verbatim in the source · abstract, characters 891–972 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The clusters were consistent with the estimates of genetic identity.

Taxon
popcorne0
Outcome
clusterse5
Qualifier
consistent with the estimates of genetic identitye5

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e5

“The clusters were consistent with the estimates of genetic identity”

✓ located verbatim in the source · abstract, characters 822–889 · show in text ↑
Recommendationhypothesisoriginal resultpositivebasis n/a

Claim · model-written summary

Higher heterozygosity is expected from crosses between lines P3-3 and P7-3 and lines P1-3 and P7-4.

Taxon
popcorne0
Material
lines P3-3 and P7-3e6
Outcome
levels of heterozygositye6
Direction
Highere6

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e6

“Higher levels of heterozygosity are expected from crosses between the group containing lines P3-3 and P7-3 with that of P1-3 and P7-4”

✓ located verbatim in the source · abstract, characters 974–1107 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Crosses between lines P1-3 and P8-1 are promising.

Taxon
popcorne0
Material
P1-3 and P8-1e7
Outcome
Crosses between linese7
Qualifier
promisinge7

Verbatim

e0

“Eight popcorn S6 lines of diverse germplasm types were evaluated using simple sequence repeats (SSR) markers”

✓ located verbatim in the source · abstract, characters 159–267 · show in text ↑
e7

“Crosses between lines P1-3 and P8-1 are also promising”

✓ located verbatim in the source · abstract, characters 1109–1163 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Popcorn breeding programmes in Brazil possibly use highly similar base-populations.

Taxon
popcorne1
Outcome
base-populationse1
Qualifier
highly similare1
Setting
Brazile1

Verbatim

e1

“The different popcorn breeding programs in Brazil are possibly using highly similar base-populations”

✓ located verbatim in the source · abstract, characters 446–546 · show in text ↑