Abstract
The tobacco N gene confers resistance to Tobacco mosaic virus (TMV) and encodes a toll-interleukin-1 receptor/nucleotide binding/Leu-rich repeat class protein. Recent evidence indicates that the Nicotiana benthamiana Rar1 gene (NbRar1), which encodes a protein with a zinc finger motif called CHORD (Cys- and His-rich domain), is required for the function of N. To investigate the role of NbRar1 in plant defense, we identified its interaction partners. We show that the NbRar1 protein interacts with NbSGT1, a highly conserved component of the SCF (Skp1/Cullin/F-box protein)-type E3 ubiquitin ligase complex involved in protein degradation. In addition, we show that NbSGT1 interacts with NbSKP1. Suppression of NbSGT1 and NbSKP1 shows that these genes play an important role in the N-mediated resistance response to TMV. Both NbRar1 and NbSGT1 associate with the COP9 signalosome, another multiprotein complex involved in protein degradation via the ubiquitin-proteasome pathway. Silencing of the NbCOP9 signalosome also compromises N-mediated resistance to TMV. Our results reveal new roles for SCF and the COP9 signalosome in plant defense signaling.
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Claims 5
Claim · model-written summary
NbRar1 interacts with NbSGT1.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Gene
- NbRar1e0
- Outcome
- interacts with NbSGT1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NbRar1 protein interacts with NbSGT1”
t0“Tobacco mosaic virus”
Claim · model-written summary
NbSGT1 interacts with NbSKP1.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- NbSGT1e0
- Outcome
- interacts with NbSKP1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbSGT1 interacts with NbSKP1”
t0“Tobacco mosaic virus”
Claim · model-written summary
Suppressing NbSGT1 and NbSKP1 impairs N-mediated resistance to TMV.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Suppression of NbSGT1 and NbSKP1e0
- Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- NbSGT1 and NbSKP1e0
- Outcome
- N-mediated resistance response to TMVe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of NbSGT1 and NbSKP1 shows that these genes play an important role in the N-mediated resistance response to TMV.”
t0“Tobacco mosaic virus”
Claim · model-written summary
NbRar1 and NbSGT1 associate with the COP9 signalosome.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NbRar1 and NbSGT1e0
- Outcome
- associate with the COP9 signalosomee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both NbRar1 and NbSGT1 associate with the COP9 signalosome”
t0“Tobacco mosaic virus”
Claim · model-written summary
Silencing the NbCOP9 signalosome compromises N-mediated TMV resistance.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Silencing of the NbCOP9 signalosomee0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Outcome
- N-mediated resistance to TMVe0
- Direction
- compromisese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of the NbCOP9 signalosome also compromises N-mediated resistance to TMV.”
t0“Tobacco mosaic virus”
The same questions in other papers
loss of function · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
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- negative
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.