Abstract
Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is the most economically important diseases of sugar beet in Iran. We have identified molecular markers associated with resistance gene(s) to this disease. A F2 population including of 106 individuals developed at the Sugar Beet Seed Institute, Karaj, Iran, was used to identify molecular markers associated with rhizomania resistance gene from the Holly source. In this study, we used pair combinations of single RAPD primers in bulked segregant analysis (BSA) of two bulks (resistant and susceptible) and F2 population individuals. Accordingly 397 pair combinations of single RAPD primers were used. However, nine primer pairs showed polymorphism between DNA bulks. The polymorphic markers were tested among the individual plants of the two susceptible and resistant bulks and further were used to analyze the F2 individuals. Finally, the markers distance from the resistance gene was estimated by using both the Map maker ver.3.0 and the frequency of recombinant plants method. Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively. On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively. After sequencing the products amplified by MF1 and MF2 (Operon primers number have been replaced by these characters) primer pairs, new PCR primers were used to generate the SCAR marker R1 (this primer sequence is under patent processing and will be shared once it gets patented) which can be readily used for marker assisted selection in breeding programmes.
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Claims 18
Claim · model-written summary
Nine primer pairs were polymorphic between resistant and susceptible DNA bulks.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarist0 - Gene
- Rz1t0
- Outcome
- primer pairs showed polymorphisme0
- Value
- ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, nine primer pairs showed polymorphism between DNA bulks.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
R1 was 2.3 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- R1e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 2.3e0
- Qualifier
- repulsion phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
R2 was 8.3 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- R2e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 8.3e0
- Qualifier
- repulsion phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
R3 was 16.6 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- R3e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 16.6 cMe0
- Qualifier
- repulsion phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C3 was 20 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C3e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 20e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C4 was 21.4 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C4e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 21.4e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C1 was 27.5 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C1e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 27.5e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C2 was 32.9 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C2e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 32.9e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C5 was 43.7 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C5e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 43.7e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C6 was 51.9 cM from Rz1 by recombinant frequency.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C6e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 51.9 cMe0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using frequency of recombinant plants method to tag Rz1 gene, it was found that R1, R2 and R3 markers were 2.3, 8.3 and 16.6 cM apart in repulsion phase and C3, C4, C1, C2, C5, and C6 markers were 20, 21.4, 27.5, 32.9, 43.7, and 51.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
R1 was 32.4 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- R1e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 32.4e0
- Qualifier
- repulsion phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
R2 was 44.5 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- R2e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 44.5e0
- Qualifier
- repulsion phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
R3 was 60.1 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- R3e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 60.1 cMe0
- Qualifier
- repulsion phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C3 was 25 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C3e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 25e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C2 was 34.7 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C2e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 34.7e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C4 was 46.5 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C4e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 46.5e0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
C1 was 57.9 cM from Rz1 by Map maker.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Material
- C1e0
- Gene
- Rz1e0
- Outcome
- markers weree0
- Value
- 57.9 cMe0
- Qualifier
- coupling phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, tagging Rz1 gene by Map maker ver.3.0 method showed that R1, R2 and R3 markers were 32.4, 44.5 and 60.1 cM apart in repulsion phase and C3, C2, C4 and C1 markers were 25, 34.7, 46.5 and 57.9 cM apart in coupling phase, respectively.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”
Claim · model-written summary
A SCAR marker R1 was generated for marker assisted selection.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- R1e0
- Outcome
- SCAR marker R1e0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After sequencing the products amplified by MF1 and MF2 (Operon primers number have been replaced by these characters) primer pairs, new PCR primers were used to generate the SCAR marker R1 (this primer sequence is under patent processing and will be shared once it gets patented) which can be readily used for marker assisted selection in breeding programmes.”
t0“Identification of SCAR and RAPD markers linked to Rz1 gene in Holly sugar beet using BSA and two genetic distance estimation methods”