Abstract
A survey of non-cultivated plants of the Tallgrass Prairie Preserve of Oklahoma for viruses used young leaf tissue for analysis of virus sequence signature content. To assess whether tissue selection may affect the results of such a survey, Snow-on-the-mountain (Euphorbia marginata Pursh) plants growing along roadsides of Payne Co., Oklahoma, were sampled during the fall of 2007. Specimens of upper leaves, lower leaves, white foliar bracts and fruits were separately processed to obtain nucleic acid preparations from virus-like particle fractions of plant homogenates. Signatures of two viruses, a comovirus and a tymovirus, Asclepias asymptomatic virus (AsAV), both also found on the Tallgrass Prairie Preserve, were observed in the sequences of the nucleic acid preparations. Not all tissues of positive plants were consistently positive. In the sequences retrieved, the nucleotides at only five positions differed from those of AsAV obtained from Asclepias viridis. Sequences from bracts and from lower leaves of different plants were more similar to one another than to other sequences from the same plant. © 2009 Oklahoma Academy of Science.
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Claims 4
Claim · model-written summary
Signatures of a comovirus and Asclepias asymptomatic virus were found in Euphorbia samples.
- Taxon
- Euphorbia marginata Pursh
Euphorbia marginatae1 - Interactor
- a comoviruse0pathogen
- Second interactor
- Asclepias asymptomatic virusAsclepias asymptomatic viruse0pathogen
- Outcome
- Signatures of two virusese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Signatures of two viruses, a comovirus and a tymovirus, Asclepias asymptomatic virus (AsAV), both also found on the Tallgrass Prairie Preserve, were observed in the sequences of the nucleic acid preparations”
e1“Snow-on-the-mountain (Euphorbia marginata Pursh) plants”
Claim · model-written summary
Not all tissues of virus positive Euphorbia plants tested positive consistently.
- Taxon
- Euphorbia marginata PurshEuphorbia marginatae1
- Organ / stage
- tissuese0
- Outcome
- Not all tissues of positive plants were consistently positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Not all tissues of positive plants were consistently positive”
e1“Snow-on-the-mountain (Euphorbia marginata Pursh) plants”
Claim · model-written summary
The retrieved sequences differed from AsAV from Asclepias viridis at only five nucleotide positions.
- Taxon
- Euphorbia marginata PurshEuphorbia marginatae1
- Interactor
- AsAVe0pathogen
- Outcome
- nucleotides at only five positions differede0
- Value
- five positionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nucleotides at only five positions differed from those of AsAV obtained from Asclepias viridis”
e1“Snow-on-the-mountain (Euphorbia marginata Pursh) plants”
Claim · model-written summary
Sequences from bracts and lower leaves of different plants were more similar than sequences from the same plant.
- Taxon
- Euphorbia marginata PurshEuphorbia marginatae1
- Organ / stage
- bracts and from lower leavese0
- Outcome
- Sequences from bracts and from lower leavese0
- Direction
- more similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequences from bracts and from lower leaves of different plants were more similar to one another than to other sequences from the same plant”
e1“Snow-on-the-mountain (Euphorbia marginata Pursh) plants”