Paper · 2011 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Inducement and identification of an endosperm mutant in maize

Ruo-Fei Yang

4
claims
7
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

60 Co gamma-ray and NaN 3. Mutant lines were derived from the regenerated plants. The results indicate that the combination of 20 Gy of gamma-ray and 1 mmol/L of NaN 3 is the most effective for mutation inducement of maize calli. Three endosperm mutant lines with “super sweet” phenotype were derived from the mutated offspring. By complementation test and DNA sequence analysis, their mutation site was found in exon 14 of gene sh2 that encodes adenosine diphosphate glucose pyrophosphorylase. For their highly consistent phenotypes of agronomic characteristics and distinguished molecular mechanism to the previous mutants of gene sh2 , these three mutant lines are regarded as a novel multiallelic mutant of gene sh2 .

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

20 Gy gamma rays with 1 mmol/L NaN 3 was most effective for mutation induction in maize calli.

Taxon
maizeZea mayse0
Treatment
20 Gy of gamma-ray and 1 mmol/L of NaN 3e0
Outcome
mutation inducement of maize callie0
Direction
most effectivee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the combination of 20 Gy of gamma-ray and 1 mmol/L of NaN 3 is the most effective for mutation inducement of maize calli”

✓ located verbatim in the source · abstract, characters 108–228 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Three endosperm mutant lines with a super sweet phenotype were derived.

Taxon
maizeZea mayst0
Material
endosperm mutant linese0
Outcome
“super sweet” phenotypee0
Value
Threee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three endosperm mutant lines with “super sweet” phenotype were derived from the mutated offspring”

✓ located verbatim in the source · abstract, characters 230–327 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The mutation site in the three lines was exon 14 of sh2.

Taxon
maizeZea mayst0
Gene
gene sh2e0
Outcome
mutation site was found in exon 14e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“their mutation site was found in exon 14 of gene sh2 that encodes adenosine diphosphate glucose pyrophosphorylase”

✓ located verbatim in the source · abstract, characters 380–493 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

The three lines were regarded as a novel multiallelic mutant of sh2.

Taxon
maizeZea mayst0
Material
three mutant linese0
Gene
gene sh2e0
Outcome
novel multiallelic mutante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“these three mutant lines are regarded as a novel multiallelic mutant of gene sh2”

✓ located verbatim in the source · abstract, characters 639–719 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 56–61 · show in text ↑

radiation · organism · Zea mays (maize)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.