Abstract
RNA isolation with RNA in a high quantity is a basic analytical method in plant genetics, molecular biology and related physiological investigations. To understand the genetic and molecular biology of Chinese fir, sufficient high-quality total RNA must be obtained for cDNA library construction and other downstream molecular applications. However, extracting RNA from Chinese fir is difficult and often requires the modification of existing protocols. Chinese fir tissues containing large amounts of polysaccharides and polyphenol compounds and are one of the most difficult plant tissues for RNA isolation. Therefore, we developed a simple method for extracting high-quality RNA from Chinese fir tissues. RNA isolations were performed within two hours, RNA quality was measured for yield and purity. Total RNA obtained from this procedure was successfully used for cDNA library construction, RT-PCR and transcriptome sequencing. It was proven that extracted RNA was intact and suitable for downstream molecular applications, including RT-PCR and qPCR, and other downstream molecular applications. Thus, this protocol represents a simple, efficient, and low-cost method.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
RNA isolation from Chinese fir tissues was completed within two hours.
Verbatim
e0“RNA isolations were performed within two hours”
t0“Chinese Fir”
Claim · model-written summary
Total RNA from Chinese fir was successfully used for cDNA library construction, RT-PCR, and transcriptome sequencing.
- Taxon
- Chinese Firt0
- Outcome
- successfully used for cDNA library construction, RT-PCR and transcriptome sequencinge0
Not reportedComparatorValue
Verbatim
e0“Total RNA obtained from this procedure was successfully used for cDNA library construction, RT-PCR and transcriptome sequencing”
t0“Chinese Fir”
Claim · model-written summary
Extracted Chinese fir RNA was intact and suitable for downstream molecular applications including RT-PCR and qPCR.
- Taxon
- Chinese Firt0
- Outcome
- extracted RNA was intact and suitable for downstream molecular applications, including RT-PCR and qPCRe0
Not reportedComparatorValue
Verbatim
e0“extracted RNA was intact and suitable for downstream molecular applications, including RT-PCR and qPCR”
t0“Chinese Fir”
Claim · model-written summary
The authors describe their Chinese fir RNA isolation protocol as simple, efficient, and low-cost.
Not reportedComparatorValue
Verbatim
e0“this protocol represents a simple, efficient, and low-cost method”
t0“Chinese Fir”