Abstract
Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to examine the effect of three cryopreservation methods (vitrification, encapsulation/vitrification and encapsulation/dehydration) and four different storage periods (0, 1, 3 and 6 months) on the survival and recovery percentages of excised in vitro- and virus-free shoot tips of two potato (Solanum tuberosum L.) cultivars, namely Spunta and Lady Rosetta. In vitro produced plantlets were pretreated with high sucrose concentrations and cold temperature (10°C), before the application of different cryopreservation methods. After pretreatments, samples were rapidly cooled in liquid nitrogen and stored at a temperature of -80°C. Cryopreserved shoot tips, after thawing for 90 seconds at 38-40°C in a water bath, were directly planted on regeneration medium for recovery. The highest survival (73.44%) as well as recovery (53.13%), of cryopreserved shoot tip explants of both potato cultivars, was observed with encapsulation/vitrification method. Increasing the storage period decreased both survival and recovery percentages of cryopreserved shoot tips. Cultivar Lady Rosetta showed superiority over Spunta, it recorded higher mean percentages of survival and recovery with encapsulation/vitrification method. Thus, this study demonstrated that encapsulation/vitrification method appears promising for cryopreservation of in vitro stored shoot tips for both cultivars of potato.
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Claims 5
Claim · model-written summary
Encapsulation/vitrification produced the highest shoot-tip survival, 73.44%.
- Taxon
- potato
Solanum tuberosume0 - Organ / stage
- shoot tip explantse0
- Treatment
- encapsulation/vitrification methode0
- Outcome
- survivale0
- Direction
- higheste0
- Value
- 73.44%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest survival (73.44%) as well as recovery (53.13%), of cryopreserved shoot tip explants of both potato cultivars, was observed with encapsulation/vitrification method”
Claim · model-written summary
Encapsulation/vitrification produced the highest shoot-tip recovery, 53.13%.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- encapsulation/vitrification methode0
- Outcome
- recoverye0
- Direction
- higheste0
- Value
- 53.13%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest survival (73.44%) as well as recovery (53.13%), of cryopreserved shoot tip explants of both potato cultivars, was observed with encapsulation/vitrification method”
Claim · model-written summary
Longer storage decreased potato shoot-tip survival and recovery.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- storage periode0
- Outcome
- survival and recovery percentagese0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the storage period decreased both survival and recovery percentages of cryopreserved shoot tips”
t0“Potato”
Claim · model-written summary
Lady Rosetta had higher survival and recovery than Spunta under encapsulation/vitrification.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- Lady Rosettae0
- Treatment
- encapsulation/vitrification methode0
- Outcome
- survival and recoverye0
- Direction
- highere0
- Comparator
- Spuntae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cultivar Lady Rosetta showed superiority over Spunta, it recorded higher mean percentages of survival and recovery with encapsulation/vitrification method”
t0“Potato”
Claim · model-written summary
The authors consider encapsulation/vitrification promising for in vitro potato shoot-tip storage.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- encapsulation/vitrification methode0
- Outcome
- cryopreservation of in vitro stored shoot tipse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encapsulation/vitrification method appears promising for cryopreservation of in vitro stored shoot tips for both cultivars of potato”
The same questions in other papers
in vitro · survival · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.