Abstract
The maintenance of Phytophthora infestans cultures isolates from blighted potato, frozen in liquid nitrogen, was studied in two experiments. The effects of deep freezing and acclimatization pre-treatment at different temperatures on culture survival and stability of virulence and aggressiveness were evaluated. The best survival of cultures maintained for three months in liquid nitrogen was expressed, when cultures were acclimatized before freezing at 7°C. The survival of frozen cultures was significantly worse in comparison with control combinations stored on rye-agar and rye-agar under paraffin oil for short time (in experiment I – 40 days, in experiment II – three months). The virulence spectrum of frozen cultures after thawing was more narrow than that one observed before culture freezing. However this virulence spectrum did not differ significantly from virulence range of control cultures. The level of aggressiveness of culture stored in liquid nitrogen did not differ significantly from control cultures as well. The virulence spectrum and aggressiveness level of cultures frozen in liquid nitrogen were significantly higher after two passages of thawed cultures on potato tissues than after single passage.
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Claims 7
Claim · model-written summary
Acclimatization at 7°C gave the best survival after three months of freezing.
- Taxon
- potato
Solanum tuberosume1 - Treatment
- acclimatized before freezing at 7°Ce0
- Interactor
- Phytophthora infestans
Phytophthora infestanst0pathogen - Outcome
- survival of culturese0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best survival of cultures maintained for three months in liquid nitrogen was expressed, when cultures were acclimatized before freezing at 7°C.”
e1“blighted potato”
t0“PHYTOPHTHORA INFESTANS”
Claim · model-written summary
Frozen culture survival was significantly worse than agar-stored controls.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- frozen culturese0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Outcome
- survival of frozen culturese0
- Direction
- worsee0
- Comparator
- control combinations stored on rye-agar and rye-agar under paraffin oile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The survival of frozen cultures was significantly worse in comparison with control combinations stored on rye-agar and rye-agar under paraffin oil for short time”
e1“blighted potato”
t0“PHYTOPHTHORA INFESTANS”
Claim · model-written summary
The virulence spectrum narrowed after freezing.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- culture freezinge0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Outcome
- virulence spectrume0
- Direction
- more narrowe0
- Comparator
- before culture freezinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virulence spectrum of frozen cultures after thawing was more narrow than that one observed before culture freezing.”
e1“blighted potato”
t0“PHYTOPHTHORA INFESTANS”
Claim · model-written summary
Frozen and control cultures did not differ significantly in virulence spectrum.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- frozen in liquid nitrogene1
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Outcome
- virulence spectrume0
- Direction
- did not differe0
- Comparator
- control culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this virulence spectrum did not differ significantly from virulence range of control cultures”
e1“blighted potato, frozen in liquid nitrogen”
t0“PHYTOPHTHORA INFESTANS”
Claim · model-written summary
Frozen and control cultures did not differ significantly in aggressiveness.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- culture stored in liquid nitrogene0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Outcome
- level of aggressivenesse0
- Direction
- did not differe0
- Comparator
- control culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of aggressiveness of culture stored in liquid nitrogen did not differ significantly from control cultures as well.”
e1“blighted potato”
t0“PHYTOPHTHORA INFESTANS”
Claim · model-written summary
Two potato-tissue passages increased the frozen isolates’ virulence spectrum relative to one passage.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- two passages of thawed cultures on potato tissuese0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Outcome
- virulence spectrume0
- Direction
- highere0
- Comparator
- single passagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virulence spectrum and aggressiveness level of cultures frozen in liquid nitrogen were significantly higher after two passages of thawed cultures on potato tissues than after single passage.”
t0“PHYTOPHTHORA INFESTANS”
Claim · model-written summary
Two potato-tissue passages increased the frozen isolates’ aggressiveness relative to one passage.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- two passages of thawed cultures on potato tissuese0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Outcome
- aggressiveness levele0
- Direction
- highere0
- Comparator
- single passagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virulence spectrum and aggressiveness level of cultures frozen in liquid nitrogen were significantly higher after two passages of thawed cultures on potato tissues than after single passage.”
t0“PHYTOPHTHORA INFESTANS”
The same questions in other papers
nitrogen · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.