Abstract
Kentucky bluegrass ( Poa pratensis L.) is an important facultative apomictic temperate perennial grass species used for both forage and cultivated turf. Through apomixis, this species is able to propagate diverse and odd ploidy levels, resulting in many genetically distinct phenotypes. A wide range of diverse cultivars and accessions of kentucky bluegrass have been previously characterized based on pedigree, common turf performance, and morphological characteristics to create a kentucky bluegrass cultivar classification system. The objectives of the current study were to assess the amount of genetic divergence among kentucky bluegrass cultivars, experimental selections, and plant collections and revise/update the original pedigree, turf performance, and morphological characteristics kentucky bluegrass classification system using recently described kentucky bluegrass microsatellite [simple sequence repeat (SSR)] markers. In this study, 247 kentucky bluegrass cultivars, experimental selections, and collections were genotyped using 25 SSR markers. SSR markers showed a strong correlation between genetic relatedness as assessed by molecular markers and the original kentucky bluegrass classification system and also provided justification for a revision/update of the classification system. Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types. Newly proposed classification types included Cynthia, Jefferson/Washington, Limousine, P-105, Sydsport, and three Eurasian types. The majority of cultivars, experimental selections, and collections were uniquely identified with the current set of SSR markers. Genetic relationships of individuals as assessed by SSR markers closely matched known pedigrees. The current set of SSR markers can be used to rapidly genotype and assign new cultivars/accessions to kentucky bluegrass classification types and assess genetic relatedness among individuals and should be considered for use in a kentucky bluegrass plant variety protection program.
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Claims 10
Claim · model-written summary
SSR markers showed a strong correlation between genetic relatedness and the original kentucky bluegrass classification system.
- Taxon
- Poa pratensis L.
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Poa pratensist0 - Outcome
- genetic relatednesse1
- Comparator
- original kentucky bluegrass classification systeme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e1“SSR markers showed a strong correlation between genetic relatedness as assessed by molecular markers and the original kentucky bluegrass classification system”
Claim · model-written summary
Most cultivars, experimental selections and collections were uniquely identified with the SSR markers.
- Taxon
- Poa pratensis L.Poa pratensist0
- Material
- cultivars, experimental selections, and collectionse2
- Outcome
- uniquely identifiede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e2“The majority of cultivars, experimental selections, and collections were uniquely identified with the current set of SSR markers”
Claim · model-written summary
BVMG, Compact and Compact-America are traditional classification types supported by the SSR analysis.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- Traditional classification types that were supported by the current SSR analysise3
- Qualifier
- BVMG, Compact, Compact-Americae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e3“Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types”
Claim · model-written summary
Julia, Mid-Atlantic and Midnight are traditional classification types supported by the SSR analysis.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- Traditional classification types that were supported by the current SSR analysise3
- Qualifier
- Julia, Mid-Atlantic, Midnighte3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e3“Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types”
Claim · model-written summary
Shamrock is a traditional classification type supported by the SSR analysis.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- Traditional classification types that were supported by the current SSR analysise3
- Qualifier
- Shamrock typese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e3“Traditional classification types that were supported by the current SSR analysis include BVMG, Compact, Compact-America, Julia, Mid-Atlantic, Midnight, and Shamrock types”
Claim · model-written summary
Cynthia, Jefferson/Washington and Limousine are newly proposed classification types.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- Newly proposed classification typese4
- Qualifier
- Cynthia, Jefferson/Washington, Limousinee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e4“Newly proposed classification types included Cynthia, Jefferson/Washington, Limousine, P-105, Sydsport, and three Eurasian types”
Claim · model-written summary
P-105, Sydsport and three Eurasian types are newly proposed classification types.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- Newly proposed classification typese4
- Qualifier
- P-105, Sydsport, and three Eurasian typese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e4“Newly proposed classification types included Cynthia, Jefferson/Washington, Limousine, P-105, Sydsport, and three Eurasian types”
Claim · model-written summary
Genetic relationships of individuals from SSR markers closely matched known pedigrees.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- Genetic relationships of individualse5
- Comparator
- known pedigreese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e5“Genetic relationships of individuals as assessed by SSR markers closely matched known pedigrees”
Claim · model-written summary
The SSR marker set can rapidly genotype and assign new cultivars and accessions to classification types.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- rapidly genotype and assign new cultivars/accessionse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e6“The current set of SSR markers can be used to rapidly genotype and assign new cultivars/accessions to kentucky bluegrass classification types and assess genetic relatedness among individuals and should be considered for use in a kentucky bluegrass plant variety protection program”
Claim · model-written summary
The SSR markers should be considered for use in a kentucky bluegrass plant variety protection program.
- Taxon
- Poa pratensis L.Poa pratensist0
- Outcome
- should be considered for use in a kentucky bluegrass plant variety protection programe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Kentucky Bluegrass (Poa pratensis L.)”
e6“The current set of SSR markers can be used to rapidly genotype and assign new cultivars/accessions to kentucky bluegrass classification types and assess genetic relatedness among individuals and should be considered for use in a kentucky bluegrass plant variety protection program”