Paper · 2010 · Pakistan Journal of Botany

GENOTYPING WITH RAPD MARKERS RESOLVES PATHOTYPE DIVERSITY IN THE ASCOCHYTA BLIGHT AND FUSARIUM WILT PATHOGENS OF CHICKPEA IN PAKISTAN

F. F. Jamil; Muhammad Kaleem Sarwar; Nighat Sarwar; et al.

5
claims
9
verbatim quotes
18
citations
3
research areas
—
Not open access

Abstract

Chickpea (Cicer arietinum L.) is an important legume in Pakistan and constitutes about 70% of the pulse crops. Unfortunately, this crop is badly affected by two diseases i.e., chickpea blight and wilt caused by the fungi Ascochyta rabiei and Fusarium oxysporum f. sp. ciceris (FOC), respectively. Pathogenicity of A. rabiei and FOC isolates collected from different areas was conducted on a set of chickpea differentials sets. Isolates of the two pathogens showing different levels of virulence were selected for their DNA fingerprinting. RAPD analysis of 12 isolates of A. rabiei and 5 of FOC was performed using sixteen different 10-mer oligonucleotide primers. The dendrograms of A. rabiei and FOC produced from this data showed different levels of genetic relatedness and differences between isolates which were superimposed by their pathotyping data. A set of 7 genetically different and highly aggressive isolates of A. rabiei from different areas was selected for thorough screening of chickpea germplasm against blight for attaining durable resistance. Similarly, the dendrogram of FOC showed that all the five isolates were very different and those from Thal area were more aggressive.

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Claims 5

Associationobservedoriginal resultmeasured

Claim · model-written summary

A. rabiei isolates differed in genetic relatedness, corresponding with pathotypes.

Taxon
CHICKPEACicer arietinumt0
Interactor
A. rabieiAscochyta rabieie0pathogen
Outcome
genetic relatednesse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Ascochyta rabiei
Ascochyta rabiei · Interactor · picture from its Wikimedia Commons categoryDr. Weidong Chen Research Plant Pathologist · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The dendrograms of A. rabiei and FOC produced from this data showed different levels of genetic relatedness and differences between isolates which were superimposed by their pathotyping data.”

✓ located verbatim in the source · abstract, characters 664–855 · show in text ↑
t0

“CHICKPEA”

✓ located verbatim in the source · title, characters 113–121 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

FOC isolates differed in genetic relatedness, corresponding with pathotypes.

Taxon
CHICKPEACicer arietinumt0
Interactor
FOCe0pathogen
Outcome
genetic relatednesse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The dendrograms of A. rabiei and FOC produced from this data showed different levels of genetic relatedness and differences between isolates which were superimposed by their pathotyping data.”

✓ located verbatim in the source · abstract, characters 664–855 · show in text ↑
t0

“CHICKPEA”

✓ located verbatim in the source · title, characters 113–121 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Seven genetically different, aggressive A. rabiei isolates were selected for chickpea blight screening.

Taxon
chickpeaCicer arietinume0
Interactor
A. rabieiAscochyta rabieie0pathogen
Outcome
selected for thorough screeninge0
Value
7e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Ascochyta rabiei
Ascochyta rabiei · Interactor · picture from its Wikimedia Commons categoryDr. Weidong Chen Research Plant Pathologist · Public domain · Wikimedia Commons ↗

Verbatim

e0

“A set of 7 genetically different and highly aggressive isolates of A. rabiei from different areas was selected for thorough screening of chickpea germplasm against blight for attaining durable resistance.”

✓ located verbatim in the source · abstract, characters 856–1060 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

All five FOC isolates were very different.

Taxon
CHICKPEACicer arietinumt0
Interactor
FOCe0pathogen
Outcome
very differente0
Value
five isolatese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Similarly, the dendrogram of FOC showed that all the five isolates were very different and those from Thal area were more aggressive.”

✓ located verbatim in the source · abstract, characters 1061–1194 · show in text ↑
t0

“CHICKPEA”

✓ located verbatim in the source · title, characters 113–121 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

FOC isolates from Thal were more aggressive.

Taxon
CHICKPEACicer arietinumt0
Interactor
FOCe0pathogen
Outcome
aggressivee0
Direction
more aggressivee0
Setting
Thal areae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Similarly, the dendrogram of FOC showed that all the five isolates were very different and those from Thal area were more aggressive.”

✓ located verbatim in the source · abstract, characters 1061–1194 · show in text ↑
t0

“CHICKPEA”

✓ located verbatim in the source · title, characters 113–121 · show in text ↑