Abstract
© 2015, Edizioni ETS. All rights reserved.An emerging soybean disease observed in Cuba was investigated in view of the possibility that a phytoplasma could be involved in its aetiology. Thirty-five soybean plants showing symptoms of stunting, chlorosis, crinkle and aborted seed pods were collected in Las Tunas and Holguin provinces during 2013, and analysed by nested- PCR with primers targeting the 16S ribosomal DNA (16S rDNA). Phytoplasmas were detected in 83% of symptomatic soybean plants. Conventional and virtual RFLP analyses of 16S rDNA sequences revealed the presence of strains of ‘Candidatus Phytoplasma asteris’ (16SrI group). Phytoplasmas belonging to ribosomal subgroup 16SrI-B and two putative new ribosomal subgroups 16SrI-W and 16SrI-Y were identified. Phylogenetic analysis corroborated the RFLP analyses, in which the Cuban strains formed a clade with representative sequences of the 16SrI group. 16SrI-B was the most prevalent subgroup (45% of positive samples) and mixed infections of different subgroups were observed (10% of the positive samples). The partial 16S rDNA sequences of three strains showed 99% nucleotide identity to several GenBank sequences of group 16SrI phytoplasmas, including a phytoplasma associated with a soybean disease in Brazil. This is the first report of a phytoplasma belonging to the 16SrI subgroup occurring in soybean in Cuba.
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Claims 8
Claim · model-written summary
Phytoplasmas were detected in 83% of symptomatic soybean plants.
- Taxon
- soybean
Glycine maxe0 - Interactor
- Phytoplasmas
Candidatus Phytoplasmae0pathogen - Outcome
- Phytoplasmas were detectede0
- Value
- 83%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phytoplasmas were detected in 83% of symptomatic soybean plants”
Claim · model-written summary
RFLP analysis identified Candidatus Phytoplasma asteris strains in soybean.
- Taxon
- soybeanGlycine maxt0
- Interactor
- Candidatus Phytoplasma asterisCandidatus Phytoplasma asterise0pathogen
- Outcome
- presence of strains of ‘Candidatus Phytoplasma asteris’ (16SrI group)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealed the presence of strains of ‘Candidatus Phytoplasma asteris’ (16SrI group)”
t0“soybean”
Claim · model-written summary
Subgroups 16SrI-B, 16SrI-W and 16SrI-Y were identified.
- Taxon
- soybeanGlycine maxt0
- Interactor
- PhytoplasmasCandidatus Phytoplasmae0pathogen
- Outcome
- ribosomal subgroup 16SrI-B and two putative new ribosomal subgroups 16SrI-W and 16SrI-Ye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phytoplasmas belonging to ribosomal subgroup 16SrI-B and two putative new ribosomal subgroups 16SrI-W and 16SrI-Y were identified”
t0“soybean”
Claim · model-written summary
Phylogenetic analysis placed Cuban strains with representative 16SrI sequences.
- Taxon
- soybeanGlycine maxt0
- Interactor
- Cuban strainse0pathogen
- Outcome
- formed a clade with representative sequences of the 16SrI groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Cuban strains formed a clade with representative sequences of the 16SrI group”
t0“soybean”
Claim · model-written summary
16SrI-B was the most prevalent subgroup, at 45% of positive samples.
- Taxon
- soybeanGlycine maxt0
- Interactor
- 16SrI-Be0pathogen
- Outcome
- 16SrI-B was the most prevalent subgroupe0
- Direction
- most prevalente0
- Value
- 45%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“16SrI-B was the most prevalent subgroup (45% of positive samples)”
t0“soybean”
Claim · model-written summary
Mixed subgroup infections occurred in 10% of positive samples.
- Taxon
- soybeanGlycine maxt0
- Interactor
- different subgroupse0pathogen
- Outcome
- mixed infections of different subgroupse0
- Value
- 10%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mixed infections of different subgroups were observed (10% of the positive samples)”
t0“soybean”
Claim · model-written summary
Three partial 16S rDNA sequences had 99% identity to group 16SrI GenBank sequences.
- Taxon
- soybeanGlycine maxt0
- Interactor
- group 16SrI phytoplasmase0pathogen
- Gene
- 16S rDNAe0
- Outcome
- nucleotide identitye0
- Value
- 99%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The partial 16S rDNA sequences of three strains showed 99% nucleotide identity to several GenBank sequences of group 16SrI phytoplasmas”
t0“soybean”
Claim · model-written summary
The authors report the first 16SrI subgroup phytoplasma occurrence in soybean in Cuba.
- Taxon
- soybeanGlycine maxe0
- Interactor
- a phytoplasmae0pathogen
- Outcome
- first report of a phytoplasma belonging to the 16SrI subgroup occurring in soybean in Cubae0
- Setting
- Cubae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is the first report of a phytoplasma belonging to the 16SrI subgroup occurring in soybean in Cuba”