Abstract
Bean rust caused by Uromyces appendiculatus and cowpea rust caused by U. vignea are important diseases of these corps in the world. Identification of these two Uromyces species has always been difficult in the past due to confusion in morphological feature and host range of the pathogens. In this study, four primers designed form the internal transcribed spacer (ITS) region of nuclear ribosomal DNA were developed for rapid and reliable identification of the two rust pathogens form Taiwan. Two of the primers, UA-ITSF and UA-ITSR, were highly specific to U. appendiculatus on common bean and the others two primers, UV-ITSF and UV-ITSR, were highly specific to U. vignae on cowpea. The four primers failed to amplify any of the eight other rust pathogens isolated from non-legume crops.
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Claims 3
Claim · model-written summary
Two of the primers, UA-ITSF and UA-ITSR, were highly specific to U. appendiculatus on common bean
- Taxon
- common bean
Phaseolus vulgarise0 - Interactor
- U. appendiculatus
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Uromyces appendiculatuse0pathogen - Outcome
- highly specific to U. appendiculatuse0
- Comparator
- U. appendiculatuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Two of the primers, UA-ITSF and UA-ITSR, were highly specific to U. appendiculatus on common bean”
Claim · model-written summary
UV-ITSF and UV-ITSR, were highly specific to U. vignae on cowpea
- Taxon
- cowpea
Vigna unguiculatae0 - Interactor
- U. vignaeUromyces vignaee0pathogen
- Outcome
- highly specific to U. vignaee0
- Comparator
- U. vignaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-ITSF and UV-ITSR, were highly specific to U. vignae on cowpea”
Claim · model-written summary
The four primers failed to amplify any of the eight other rust pathogens isolated from non-legume crops
Verbatim
e0“The four primers failed to amplify any of the eight other rust pathogens isolated from non-legume crops”