Abstract
BACKGROUND: Fusarium oxysporum is one of the most common fungal pathogens causing soybean root rot and seedling blight in U.S.A. In a recent study, significant variation in aggressiveness was observed among isolates of F. oxysporum collected from roots in Iowa, ranging from highly pathogenic to weakly or non-pathogenic isolates. RESULTS: We used RNA-seq analysis to investigate the molecular aspects of the interactions of a partially resistant soybean genotype with non-pathogenic/pathogenic isolates of F. oxysporum at 72 and 96 h post inoculation (hpi). Markedly different gene expression profiles were observed in response to the two isolates. A peak of highly differentially expressed genes (HDEGs) was triggered at 72 hpi in soybean roots and the number of HDEGs was about eight times higher in response to the pathogenic isolate compared to the non-pathogenic one (1,659 vs. 203 HDEGs, respectively). Furthermore, the magnitude of induction was much greater in response to the pathogenic isolate. This response included a stronger activation of defense-related genes, transcription factors, and genes involved in ethylene biosynthesis, secondary and sugar metabolism. CONCLUSIONS: The obtained data provide an important insight into the transcriptional responses of soybean-F. oxysporum interactions and illustrate the more drastic changes in the host transcriptome in response to the pathogenic isolate. These results may be useful in the developing new methods of broadening resistance of soybean to F. oxysporum, including the over-expression of key soybean genes.
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Claims 5
Claim · model-written summary
Soybean roots had markedly different gene expression profiles in response to the two Fusarium isolates.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- rootst0
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporumt0pathogen - Outcome
- gene expression profilese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“Markedly different gene expression profiles were observed in response to the two isolates”
t0“soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum”
Claim · model-written summary
At 72 hpi, soybean roots had about eight times more highly differentially expressed genes in response to the pathogenic isolate than the non-pathogenic isolate.
- Taxon
- soybeanGlycine maxt0
- Treatment
- pathogenic isolatee0
- Interactor
- Fusarium oxysporumFusarium oxysporumt0pathogen
- Outcome
- number of HDEGse0
- Direction
- highere0
- Value
- about eight timese0
- Qualifier
- 1,659 vs. 203 HDEGse0
- Comparator
- non-pathogenic onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum”
e0“the number of HDEGs was about eight times higher in response to the pathogenic isolate compared to the non-pathogenic one (1,659 vs. 203 HDEGs, respectively)”
Claim · model-written summary
The magnitude of soybean gene induction was greater with the pathogenic isolate.
- Taxon
- soybeanGlycine maxt0
- Treatment
- pathogenic isolatee0
- Interactor
- Fusarium oxysporumFusarium oxysporumt0pathogen
- Outcome
- magnitude of inductione0
- Direction
- greatere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum”
e0“the magnitude of induction was much greater in response to the pathogenic isolate”
Claim · model-written summary
The pathogenic isolate elicited stronger activation of defense-related genes, transcription factors and genes involved in ethylene biosynthesis.
- Taxon
- soybeanGlycine maxt0
- Treatment
- pathogenict0
- Interactor
- Fusarium oxysporumFusarium oxysporumt0pathogen
- Outcome
- defense-related genes, transcription factors, and genes involved in ethylene biosynthesise0
- Direction
- strongere0
- Comparator
- non-pathogenict0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum”
e0“a stronger activation of defense-related genes, transcription factors, and genes involved in ethylene biosynthesis, secondary and sugar metabolism”
Claim · model-written summary
The pathogenic isolate elicited stronger activation of genes involved in secondary and sugar metabolism.
- Taxon
- soybeanGlycine maxt0
- Treatment
- pathogenict0
- Interactor
- Fusarium oxysporumFusarium oxysporumt0pathogen
- Outcome
- secondary and sugar metabolisme0
- Direction
- strongere0
- Comparator
- non-pathogenict0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum”
e0“a stronger activation of defense-related genes, transcription factors, and genes involved in ethylene biosynthesis, secondary and sugar metabolism”