Abstract
Protoplast fusion provides a nonsexual system for the transfer of genetic information between cell types. This transfer can be between species, genera, families, or kingdoms, thereby allowing unique opportunities to study somatic cell genetics in plants. Individual chromosomes () or pieces of chromosomes () have been transferred between species in unstable hybrids, but the technology also allows the transfer and recombination of organelles (). The major obstacle in most protoplast fusion experiments has been developing strategies for the selection of somatic hybrids. This is mainly because of the fact that the fusion process itself is random. The isolation of heterokaryons from homokaryons and unfused protoplasts requires the use of a selection method. There are many selection strategies available (), but one of the most efficient methods employs the use of resistance complementation ().
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Claims 3
Claim · model-written summary
Protoplast fusion can transfer chromosomes or chromosome pieces between species.
- Taxon
- plantsViridiplantaee1
- Outcome
- chromosomes () or pieces of chromosomes () have been transferrede0
Verbatim
e0“Individual chromosomes () or pieces of chromosomes () have been transferred between species in unstable hybrids”
t0“Somatic Hybrids”
e1“somatic cell genetics in plants”
Claim · model-written summary
Protoplast fusion permits transfer and recombination of organelles.
- Taxon
- plantsViridiplantaee1
- Outcome
- transfer and recombination of organellese0
Verbatim
e0“the technology also allows the transfer and recombination of organelles”
t0“Somatic Hybrids”
e1“somatic cell genetics in plants”
Claim · model-written summary
Resistance complementation is described as one of the most efficient selection strategies.
- Taxon
- plantsViridiplantaee1
- Treatment
- resistance complementatione0
- Outcome
- most efficient methodse0
Verbatim
e0“one of the most efficient methods employs the use of resistance complementation”
t0“Somatic Hybrids”
e1“somatic cell genetics in plants”