Abstract
ABSTRACT Morphological and molecular techniques were used to evaluate the characteristics of 12 mango ( Mangifera indica L. ) varieties cultivated in Mauritius and to compare their genetic relatedness. Genomic DNA was extracted from leaf samples and genetic characterization was carried out using the RAPD technique making use of 30 decamer Operon primers. 77.1% polymorphism was obtained with Operon primer OPD-2 being the least discriminatory in contrast to OPA-9 and OPL-14, which were most polymorphic. An Un-weighted Pair Group Method with Arithmetic Mean dendogram of cophenetic ratio 0.9732 was constructed with distance values of 0.222 to 0.667. Two major clusters consisting of a mixture of foreign and native mango varieties were delimited with Overseer being the least related to the rest of the varieties. No clear distinction between the landraces and exotic varieties was observed. Phenotypically similar Jose and Eugenie varieties were also genetically close. The 3 ‘pickle’ varieties Dauphine, Sabre and Blanc were within the same cluster. Considerable genetic variation was observed between the studied mango varieties. The RAPD technique together with the analysis of morphological characters proved useful for assessing the diversity existing between the mango varieties. In conclusion, the mango varieties used in this study could be tapped in breeding programs aimed at cu ltivar improvement. © 2011 Friends Science Publishers
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Claims 11
Claim · model-written summary
RAPD analysis yielded 77.1% polymorphism, with OPD-2 least discriminatory and OPA-9 and OPL-14 most polymorphic.
- Taxon
- mango
_from_India.jpg?width=60)
Mangifera indicat0 - Outcome
- polymorphisme0
- Value
- 77.1%e0
- Qualifier
- Operon primer OPD-2 being the least discriminatory in contrast to OPA-9 and OPL-14, which were most polymorphice0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_from_India.jpg?width=500)
Verbatim
e0“77.1% polymorphism was obtained with Operon primer OPD-2 being the least discriminatory in contrast to OPA-9 and OPL-14, which were most polymorphic.”
t0“mango (Mangifera indica)”
Claim · model-written summary
The constructed dendrogram had cophenetic ratio 0.9732.
- Taxon
- mangoMangifera indicat0
- Outcome
- cophenetic ratioe0
- Value
- 0.9732e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An Un-weighted Pair Group Method with Arithmetic Mean dendogram of cophenetic ratio 0.9732 was constructed with distance values of 0.222 to 0.667.”
t0“mango (Mangifera indica)”
Claim · model-written summary
Genetic distance values ranged from 0.222 to 0.667.
- Taxon
- mangoMangifera indicat0
- Outcome
- distance valuese0
- Value
- 0.222 to 0.667e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An Un-weighted Pair Group Method with Arithmetic Mean dendogram of cophenetic ratio 0.9732 was constructed with distance values of 0.222 to 0.667.”
t0“mango (Mangifera indica)”
Claim · model-written summary
Two major clusters mixed foreign and native mango varieties.
- Taxon
- mangoMangifera indicae0
- Outcome
- major clusters consisting of a mixture of foreign and native mango varietiese0
- Value
- Twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two major clusters consisting of a mixture of foreign and native mango varieties were delimited with Overseer being the least related to the rest of the varieties.”
Claim · model-written summary
Overseer was least related to the other mango varieties.
- Taxon
- mangoMangifera indicae0
- Material
- Overseere0
- Outcome
- relatede0
- Direction
- leaste0
- Comparator
- rest of the varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two major clusters consisting of a mixture of foreign and native mango varieties were delimited with Overseer being the least related to the rest of the varieties.”
Claim · model-written summary
Landraces and exotic varieties were not clearly distinguished.
- Taxon
- mangoMangifera indicat0
- Outcome
- No clear distinction between the landraces and exotic varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No clear distinction between the landraces and exotic varieties was observed.”
t0“mango (Mangifera indica)”
Claim · model-written summary
Phenotypically similar Jose and Eugenie were genetically close.
- Taxon
- mangoMangifera indicat0
- Material
- Jose and Eugeniee0
- Outcome
- genetically closee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenotypically similar Jose and Eugenie varieties were also genetically close.”
t0“mango (Mangifera indica)”
Claim · model-written summary
Dauphine, Sabre and Blanc pickle varieties clustered together.
- Taxon
- mangoMangifera indicat0
- Material
- Dauphine, Sabre and Blance0
- Outcome
- within the same clustere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 3 ‘pickle’ varieties Dauphine, Sabre and Blanc were within the same cluster.”
t0“mango (Mangifera indica)”
Claim · model-written summary
Considerable genetic variation occurred among the mango varieties.
- Taxon
- mangoMangifera indicae0
- Outcome
- genetic variatione0
- Qualifier
- Considerablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Considerable genetic variation was observed between the studied mango varieties.”
Claim · model-written summary
RAPD with morphological analysis was useful for assessing mango variety diversity.
- Taxon
- mangoMangifera indicae0
- Outcome
- assessing the diversity existing between the mango varietiese0
- Qualifier
- usefule0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RAPD technique together with the analysis of morphological characters proved useful for assessing the diversity existing between the mango varieties.”
Claim · model-written summary
The mango varieties could be used in breeding for cultivar improvement.
- Taxon
- mangoMangifera indicae0
- Outcome
- breeding programs aimed at cu ltivar improvemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mango varieties used in this study could be tapped in breeding programs aimed at cu ltivar improvement”