Abstract
Seed quality and seed cost are the key competing factors in this rapidly globalizing seed markets thereby emphasizing the role of seed testing for seed quality assurance. Conventionally, hybrid seed purity assessment is performed through grow out test (GOT), which is highly land and labour intensive and environmentally influenced. The purity of hybrid rice (Oryza sativa L.) was tested by polymerase chain reaction (PCR) assay-based on simple sequence repeat (SSR) markers in F1 hybrids, viz. KRH-2 and DRRH-2 along with their parental lines. Among thirty five SSR markers screened for polymorphism, four pairs of SSR markers, viz. RM 206 and RM 276 for hybrid rice KRH-2 and RM 204 and RM 228 for hybrid rice DRRH-2 were identified to be polymorphic that could distinguish the hybrids from their parental lines. These SSR markers showed both alleles of the parental lines in pure hybrids proving the heterozygosity, that could also effectively identify the off-types and the selfed seeds from its respective F1 hybrid seeds using these SSR primers, this was on par with the results recorded from field GOT tests. Thus, successful in establishing four SSR markers RM 206 and RM 276 and RM 204 and RM 228 unique forthe tworice hybrids KRH-2 and DRRH-2 along with their parental lines, respectively. The high discriminating power and reliable PCR assays based SSR markers is cost effective capable of replacing the conventional GOT. These molecular markers, in addition to benefiting the seed industry to a greater extent, proves to be very important for plant improvement, variety registration system, DUS testing, seed purity testing and Protection of Plant Varieties and Farmers Rights (PPV&FR).
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Claims 6
Claim · model-written summary
RM 206 and RM 276 distinguished KRH-2 hybrids from parental lines.
- Taxon
- hybrid rice
Oryza sativae0 - Material
- KRH-2e0
- Gene
- RM 206 and RM 276e0
- Outcome
- distinguish the hybrids from their parental linese0
- Value
- four pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four pairs of SSR markers, viz. RM 206 and RM 276 for hybrid rice KRH-2 and RM 204 and RM 228 for hybrid rice DRRH-2 were identified to be polymorphic that could distinguish the hybrids from their parental lines”
Claim · model-written summary
RM 204 and RM 228 distinguished DRRH-2 hybrids from parental lines.
- Taxon
- hybrid riceOryza sativae0
- Material
- DRRH-2e0
- Gene
- RM 204 and RM 228e0
- Outcome
- distinguish the hybrids from their parental linese0
- Value
- four pairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four pairs of SSR markers, viz. RM 206 and RM 276 for hybrid rice KRH-2 and RM 204 and RM 228 for hybrid rice DRRH-2 were identified to be polymorphic that could distinguish the hybrids from their parental lines”
Claim · model-written summary
The SSR markers showed parental alleles in pure hybrids, confirming heterozygosity.
- Taxon
- hybrid riceOryza sativat0
- Outcome
- both alleles of the parental lines in pure hybridse0
- Qualifier
- heterozygositye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These SSR markers showed both alleles of the parental lines in pure hybrids proving the heterozygosity”
t0“hybrid rice”
Claim · model-written summary
The SSR assay identified off-types and selfed seeds from F1 hybrid seed lots.
- Taxon
- hybrid riceOryza sativat0
- Outcome
- identify the off-types and the selfed seedse0
- Comparator
- F1 hybrid seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could also effectively identify the off-types and the selfed seeds from its respective F1 hybrid seeds using these SSR primers”
t0“hybrid rice”
Claim · model-written summary
SSR purity results were on par with field grow-out tests.
- Taxon
- hybrid riceOryza sativat0
- Outcome
- on par with the resultse0
- Direction
- on pare0
- Comparator
- field GOT testse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this was on par with the results recorded from field GOT tests”
t0“hybrid rice”
Claim · model-written summary
The authors judged the SSR assays cost effective and suitable to replace conventional grow-out tests.
- Taxon
- hybrid riceOryza sativat0
- Outcome
- replacing the conventional GOTe0
- Qualifier
- cost effectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high discriminating power and reliable PCR assays based SSR markers is cost effective capable of replacing the conventional GOT”
t0“hybrid rice”