Abstract
We have cloned and sequenced a number of auxin-responsive cDNAs and their corresponding genes from soybean and Arabidopsis. Each of these genes, with the exception of GH2/4, is transcriptionally regulated specifically by auxins within minutes after hormone application. The auxin-responsive mRNAs are induced some 3-60-fold depending on the type of mRNA analysed, the tissue examined, the dose and duration of auxin application, and the manipulation of the organ tested. Some of the mRNAs show rapid turnover kinetics. The mRNAs show distinct patterns of organ-specific, tissue-specific, and developmental-specific expression. The promoters of the auxin-responsive genes have been fused to the E. coli uidA gene which encodes β-glucuronidase (GUS) and transferred into tobacco and/or Arabidopsis via Agrobacterium T-DNA. These promoters and parts of these promoters have been used to follow the expression patterns and auxin-inducibility of the reporter genes in transgenic plants. We are attempting to identify minimal auxin-responsive elements and gravity-responsive elements within these promoters. We have also fused the auxin-inducible promoters to bacterial genes that encode cytokinin and auxin biosynthetic or conjugating enzymes to study the effects of organ, tissue, and developmental-specific expression of cytokinins and auxins on plant growth, development, and physiology.
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Claims 5
Claim · model-written summary
Auxin-responsive cDNAs and corresponding genes were cloned and sequenced from soybean and Arabidopsis.
- Taxon
- soybean and Arabidopsis
Arabidopsis
Glycine maxe0 - Outcome
- auxin-responsive cDNAs and their corresponding genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cloned and sequenced a number of auxin-responsive cDNAs and their corresponding genes from soybean and Arabidopsis”
Claim · model-written summary
Except GH2/4, these genes were specifically transcriptionally regulated by auxins within minutes.
- Taxon
- soybean and ArabidopsisArabidopsisGlycine maxe1
- Treatment
- auxinse0
- Gene
- GH2/4e0
- Outcome
- transcriptionally regulatede0
- Qualifier
- within minutes after hormone applicatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Each of these genes, with the exception of GH2/4, is transcriptionally regulated specifically by auxins within minutes after hormone application”
e1“from soybean and Arabidopsis”
Claim · model-written summary
Auxin-responsive mRNAs were induced 3-60-fold, depending on mRNA type, tissue, dose, duration and organ manipulation.
- Taxon
- soybean and ArabidopsisArabidopsisGlycine maxe1
- Treatment
- auxin applicatione0
- Outcome
- auxin-responsive mRNAse0
- Direction
- inducede0
- Value
- 3-60-folde0
- Qualifier
- depending on the type of mRNA analysed, the tissue examined, the dose and duration of auxin application, and the manipulation of the organ testede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin-responsive mRNAs are induced some 3-60-fold depending on the type of mRNA analysed, the tissue examined, the dose and duration of auxin application, and the manipulation of the organ tested”
e1“from soybean and Arabidopsis”
Claim · model-written summary
Some auxin-responsive mRNAs had rapid turnover.
- Taxon
- soybean and ArabidopsisArabidopsisGlycine maxe1
- Outcome
- rapid turnover kineticse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the mRNAs show rapid turnover kinetics”
e1“from soybean and Arabidopsis”
Claim · model-written summary
Auxin-responsive mRNAs had distinct organ, tissue and developmental expression patterns.
- Taxon
- soybean and ArabidopsisArabidopsisGlycine maxe1
- Outcome
- distinct patterns of organ-specific, tissue-specific, and developmental-specific expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mRNAs show distinct patterns of organ-specific, tissue-specific, and developmental-specific expression”
e1“from soybean and Arabidopsis”
The same questions in other papers
class · physiology: hormones · Glycine max (soybean)Positive and negative results
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- negative
- negative
- no effect
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See all 6 papers →class · physiology: hormones · ArabidopsisPositive and negative results
6 papers in the same direction as this paper · 7 in the opposite direction
- no effect
- no effect
- no effect
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.