Abstract
Multistrain mixtures of biocontrol agents which can reduce plant disease to a greater extent than the individual strains of the mixture, commonly, are prepared by blending separately produced fermentation products. Co-cultivation of strains to equivalent biomass yields would provide mixture advantages without incurring the cost disadvantages of multiple fermentation and processing protocols. Fusariwn Head Blight (FHB) antagonists Cryptococcus flavescens OR 182.9 (NRRL Y-302l6), C. aureus OH 181.1 (NRRL Y-302l5) and C. aureus OR 181.1 (NRRL Y -30215), were grown in two- and three-strain co-cultures to assess the quality and efficacy of the fermentation end products produced. Final cell counts of component strains of all co-cultures produced were equivalent when plated on a medilllll that contained the trisaccharide melezitose as a sole carbon source and produced colonies of strain-distinguishable sizes. Co-cultures of C. flavescens OH 182.9 and C. aureus OH 71.4 significantly reduced FHB disease severity (32%, p = 0.05, Dunnett's t-test) when averaged across four greenhouse studies. In wheat field trials, biomass from co-cultures of these two strains reduced FHB incidence in some cases but rarely other FHB disease parameters (p = 0.05, Bonferoni mean separation). Relative Performance Index (RPI) analysis of the overall effect of treatments at both field sites revealed that treatment with the OH 71.4 and OH 182.9 co-culture significantly reduced FHB, as evidence by a higher RPI value than for the control, while the individual strains did not. The potential for obtaining superior efficacy and cost benefits with multi-strain cultures of biocontrol agents justifies additional research effort.
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Claims 6
Claim · model-written summary
Component strains in all co-cultures yielded equivalent final cell counts.
- Taxon
- Wheat
Triticum aestivumt0 - Outcome
- Final cell counts of component strainse0
- Direction
- equivalente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Final cell counts of component strains of all co-cultures produced were equivalent”
t0“Wheat”
Claim · model-written summary
A co-culture of C. flavescens OH 182.9 and C. aureus OH 71.4 reduced FHB severity by 32% in greenhouse studies.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Co-cultures of C. flavescens OH 182.9 and C. aureus OH 71.4e0
- Interactor
- C. flavescens OH 182.9e0biocontrol_agent
- Second interactor
- C. aureus OH 71.4Papiliotrema aureae0biocontrol_agent
- Outcome
- FHB disease severitye0
- Direction
- reducede0
- Value
- 32%e0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-cultures of C. flavescens OH 182.9 and C. aureus OH 71.4 significantly reduced FHB disease severity (32%, p = 0.05, Dunnett's t-test) when averaged across four greenhouse studies”
t0“Wheat”
Claim · model-written summary
The two-strain biomass reduced FHB incidence in some wheat field trials.
- Taxon
- wheatTriticum aestivume0
- Treatment
- biomass from co-cultures of these two strainse0
- Outcome
- FHB incidencee0
- Direction
- reducede0
- Qualifier
- in some casese0
- Setting
- field trialse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In wheat field trials, biomass from co-cultures of these two strains reduced FHB incidence in some cases”
Claim · model-written summary
The same field treatment rarely reduced other FHB parameters.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- other FHB disease parameterse0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but rarely other FHB disease parameters (p = 0.05, Bonferoni mean separation)”
t0“Wheat”
Claim · model-written summary
RPI analysis found significantly reduced FHB with the co-culture relative to control.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- treatment with the OH 71.4 and OH 182.9 co-culturee0
- Outcome
- FHBe0
- Direction
- reducede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with the OH 71.4 and OH 182.9 co-culture significantly reduced FHB, as evidence by a higher RPI value than for the control”
t0“Wheat”
Claim · model-written summary
Individual strains did not significantly reduce FHB in the RPI analysis.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- the individual strainse0
- Direction
- did note0
Not reportedComparatorOutcome
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while the individual strains did not”
t0“Wheat”