Abstract
This study was conducted to establish an efficient screening system for resistant tomato to bacterial wilt caused by Ralstonia solanacearum. Under several conditions such as inoculation methods, growth stages of tomato seedlings, inoculum concentrations, and incubating temperatures after inoculation, development of bacterial wilt on nine resistant or susceptible cultivars of tomato was investigated. To inoculate by drenching the non-cut roots with the bacterial suspension was better to distinguish resistance and susceptibility of tomato cultivars than by drenching the cut roots using scalpel. And ‘Hawaii7996’ a resistant tomato to R. solanacearum showed high resistance at all the tested conditions including growth stages (3-, 6-, 8-, 10-leaf stages), inoculum concentrations (OD600=0.1-0.4) and incubation temperatures (25, 30, 35°C). On the other hands, susceptible cultivars represented disease index of 3.7 and 3.9 at 6- and 8-leaf stages, respectively. At 3- and 10-leaf stages, the cultivars demonstrated lower disease severity of 2.1 and 0.5, respectively, than at 6- and 8-leaf stages. When the inoculated seedlings were incubated in growth chambers of 25, 30 and 35°C, disease severity of susceptible cultivars was significantly greater at 30 and 35°C than at 25°C. In addition, the level of resistance of the tomato cultivars was not significantly affected by inoculum concentrations of OD600=0.1–0.4. On the basis of the results, we suggest an efficient screening method to measure resistance level of tomato cultivars to bacterial wilt. The eight-leaf stage seedlings transplanted 7 days before inoculation, are inoculated with R. solanacearum by drenching the non-cut roots with a bacterial suspensions (OD600=0.4) to give inoculum volume of 50 ml/soil l. The inoculated plants are incubated in a growth room at 30°C for 12-13 days with 12-hour light a day.
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Claims 8
Claim · model-written summary
Drenching uncut tomato roots distinguished resistant and susceptible cultivars better than drenching cut roots.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- drenching the non-cut rootse0
- Outcome
- distinguish resistance and susceptibilitye0
- Direction
- bettere0
- Value
- non-cut rootse0
- Comparator
- drenching the cut roots using scalpele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“To inoculate by drenching the non-cut roots with the bacterial suspension was better to distinguish resistance and susceptibility of tomato cultivars than by drenching the cut roots using scalpel.”
Claim · model-written summary
Hawaii7996 showed high resistance under all tested seedling stages, inoculum concentrations and temperatures.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Hawaii7996e0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Outcome
- resistancee0
- Direction
- high resistancee0
- Qualifier
- all the tested conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“‘Hawaii7996’ a resistant tomato to R. solanacearum showed high resistance at all the tested conditions including growth stages (3-, 6-, 8-, 10-leaf stages), inoculum concentrations (OD600=0.1-0.4) and incubation temperatures (25, 30, 35°C)”
Claim · model-written summary
Susceptible cultivars had disease indices of 3.7 and 3.9 at six and eight leaves.
- Material
- susceptible cultivarse0
- Organ / stage
- 6- and 8-leaf stagese0
- Outcome
- disease indexe0
- Value
- 3.7 and 3.9e0
Not reportedTaxon
Verbatim
e0“susceptible cultivars represented disease index of 3.7 and 3.9 at 6- and 8-leaf stages, respectively”
Claim · model-written summary
Susceptible cultivars had a disease severity of 2.1 at the three-leaf stage.
Not reportedTaxon
Verbatim
e0“At 3- and 10-leaf stages, the cultivars demonstrated lower disease severity of 2.1 and 0.5, respectively, than at 6- and 8-leaf stages.”
Claim · model-written summary
Susceptible cultivars had a disease severity of 0.5 at the ten-leaf stage.
Not reportedTaxon
Verbatim
e0“At 3- and 10-leaf stages, the cultivars demonstrated lower disease severity of 2.1 and 0.5, respectively, than at 6- and 8-leaf stages.”
Claim · model-written summary
Disease severity of susceptible cultivars was significantly greater at 30 and 35°C than at 25°C.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- susceptible cultivarse0
- Treatment
- 30 and 35°Ce0
- Outcome
- disease severitye0
- Direction
- greatere0
- Comparator
- 25°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease severity of susceptible cultivars was significantly greater at 30 and 35°C than at 25°C”
t0“Tomato Cultivars”
Claim · model-written summary
Tomato cultivar resistance was not significantly affected across inoculum concentrations of OD600=0.1–0.4.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- inoculum concentrations of OD600=0.1–0.4e0
- Outcome
- level of resistancee0
- Direction
- not significantly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the level of resistance of the tomato cultivars was not significantly affected by inoculum concentrations of OD600=0.1–0.4”
Claim · model-written summary
The authors recommend screening eight-leaf tomato seedlings seven days after transplanting by drenching intact roots with R. solanacearum at OD600=0.4, then incubating at 30°C for 12–13 days.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- eight-leaf stage seedlingse0
- Treatment
- R. solanacearume0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Outcome
- drenching the non-cut roots with a bacterial suspensionse0
- Setting
- growth roome0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The eight-leaf stage seedlings transplanted 7 days before inoculation, are inoculated with R. solanacearum by drenching the non-cut roots with a bacterial suspensions (OD600=0.4) to give inoculum volume of 50 ml/soil l. The inoculated plants are incubated in a growth room at 30°C for 12-13 days with 12-hour light a day.”
t0“Tomato Cultivars”
The same questions in other papers
inoculation · disease · Solanum lycopersicum (tomato)Positive and negative results
129 papers in the same direction as this paper · 136 in the opposite direction
- positive
- positive
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