Abstract
Improvements are reported in the Raggio technique (e.g. Barrios, Raggio, and Raggio, 1963) for producing nodules on explants of roots excised from seedlings of Phaseolus vulgaris and grown in sterile culture. The principal modification is the inclusion of a portion of the hypocotyl in the explant. In a factorial experiment testing also seed size, germination temperature, and size of seedling at the time the explant was taken, the number of nodules formed was increased in this way from an average of 0·6 to around 12 per explant, and the largest number obtained was 35; without the piece of hypocotyl the largest number of nodules was 3 per explant.
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Claims 2
Claim · model-written summary
Including a portion of the hypocotyl in the explant increased nodule number per explant to around 12.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarise1 - Treatment
- inclusion of a portion of the hypocotyl in the explante2
- Outcome
- number of nodules formede4
- Direction
- increasede4
- Value
- around 12 per explante4
- Comparator
- without the piece of hypocotyle5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“producing nodules on explants of roots excised from seedlings of Phaseolus vulgaris”
e2“The principal modification is the inclusion of a portion of the hypocotyl in the explant”
e4“the number of nodules formed was increased in this way from an average of 0·6 to around 12 per explant”
e5“the largest number obtained was 35; without the piece of hypocotyl the largest number of nodules was 3 per explant”
Claim · model-written summary
The largest nodule number per explant was higher with a hypocotyl piece (35) than without it (3).
- Taxon
- Phaseolus vulgarisPhaseolus vulgarise1
- Treatment
- inclusion of a portion of the hypocotyl in the explante2
- Outcome
- largest number of nodulese5
- Direction
- higherinferrede5
- Value
- 35e5
- Comparator
- without the piece of hypocotyle5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“producing nodules on explants of roots excised from seedlings of Phaseolus vulgaris”
e2“The principal modification is the inclusion of a portion of the hypocotyl in the explant”
e5“the largest number obtained was 35; without the piece of hypocotyl the largest number of nodules was 3 per explant”
The same questions in other papers
in vitro · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.