Abstract
It was an effective method to clone NBS using the degenerate primers designed by the conserved motifs of NBS of the cloned R genes.Three sequences were cloned from wild rice HLW028 and identified as NBS.The accession numbers were DQ272573—DQ272575.The NBS of wild rice and the functionally known NBS of rice were downloaded.Phylogenetic analyses were conducted with the three submitted NBS and other downloaded NBS.The NBS could be divided into five groups,DQ272574 was a new kind of NBS.The submitted NBS contained the motifs such as P-loop,RNBS-A,kin-2,RNBS-B,RNBS-C and PLAL.RT-PCR indicated that the expression of Pikh in Peiai64 was higher than that in 2 wild rice.A cDNA with intact open reading frame was amplified from Peiai64.
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Claims 7
Claim · model-written summary
Degenerate primers effectively cloned NBS sequences.
- Taxon
- rice
Oryza sativat0 - Outcome
- effective method to clone NBSe0
- Comparator
- conserved motifs of NBSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was an effective method to clone NBS using the degenerate primers designed by the conserved motifs of NBS of the cloned R genes”
t0“rice”
Claim · model-written summary
Three NBS sequences were cloned from wild rice HLW028.
Verbatim
e0“Three sequences were cloned from wild rice HLW028 and identified as NBS”
Claim · model-written summary
Phylogenetic analysis divided NBS into five groups.
- Taxon
- riceOryza sativat0
- Gene
- NBSe0
- Outcome
- NBS could be divided into five groupse0
- Value
- five groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NBS could be divided into five groups”
t0“rice”
Claim · model-written summary
DQ272574 was identified as a new NBS type.
- Taxon
- riceOryza sativat0
- Gene
- DQ272574e0
- Outcome
- new kind of NBSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DQ272574 was a new kind of NBS”
t0“rice”
Claim · model-written summary
Submitted NBS contained P-loop, RNBS-A, kin-2, RNBS-B, RNBS-C and PLAL motifs.
- Taxon
- riceOryza sativat0
- Gene
- NBSe0
- Outcome
- motifs such as P-loop,RNBS-A,kin-2,RNBS-B,RNBS-C and PLALe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The submitted NBS contained the motifs such as P-loop,RNBS-A,kin-2,RNBS-B,RNBS-C and PLAL”
t0“rice”
Claim · model-written summary
Pikh expression in Peiai64 exceeded that in two wild rice plants.
Verbatim
e0“the expression of Pikh in Peiai64 was higher than that in 2 wild rice”
Claim · model-written summary
An intact open-reading-frame cDNA was amplified from Peiai64.
- Taxon
- riceOryza sativat0
- Material
- Peiai64e0
- Outcome
- cDNA with intact open reading framee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA with intact open reading frame was amplified from Peiai64”
t0“rice”