Abstract
DNA barcoding is a promising tool for species identification at the molecular level. The barcoding system is well established for species differentiation in animals, while it is less common in plants. We evaluated 2 barcoding regions, maturase K (matK) and ribulose bisphosphate carboxylase (rbcL), to compare species of Palmae according to amplification success, discrimination power, and inter- and intra-specific divergence. Both regions appear to have potential to discriminate most species of Palmae, but 2 species, Phoenix dactylifera and Phoenix sylvestris, did not show variation in the nucleotides of the barcode genes. P. sylvestris is said to be the sister species of P. dactilyfera according to its morphological and genetic proximity to the cultivated date palm. Thus, the status of these 2 species needs to be re-evaluated considering more genes as barcodes. Furthermore, rbcL has a higher discrimination power (90%) than matK (66.6%) and can thus be potentially used as a standard barcode to discriminate the species of Palmae.
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Claims 6
Claim · model-written summary
Both matK and rbcL had potential to discriminate most Palmae species.
- Outcome
- discriminate most speciese0
Not reportedComparator
Verbatim
e0“Both regions appear to have potential to discriminate most species of Palmae”
Claim · model-written summary
Phoenix dactylifera and Phoenix sylvestris lacked nucleotide variation in barcode genes.
- Taxon
- Phoenix dactylifera and Phoenix sylvestris
Phoenix dactylifera
Phoenix sylvestrise0 - Outcome
- variation in the nucleotides of the barcode genese0
- Direction
- did not showe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2 species, Phoenix dactylifera and Phoenix sylvestris, did not show variation in the nucleotides of the barcode genes.”
Claim · model-written summary
rbcL discrimination power was 90%.
Verbatim
e0“rbcL has a higher discrimination power (90%) than matK (66.6%)”
t0“DNA barcoding for species identification in the Palmae family”
Claim · model-written summary
matK discrimination power was 66.6%, lower than rbcL.
Not reportedDirection
Verbatim
e0“rbcL has a higher discrimination power (90%) than matK (66.6%)”
t0“DNA barcoding for species identification in the Palmae family”
Claim · model-written summary
The authors recommend re-evaluating Phoenix dactylifera and P. sylvestris status using more barcode genes.
- Taxon
- Phoenix dactylifera and Phoenix sylvestrisPhoenix dactyliferaPhoenix sylvestrise1
- Outcome
- status of these 2 speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the status of these 2 species needs to be re-evaluated considering more genes as barcodes.”
e1“2 species, Phoenix dactylifera and Phoenix sylvestris, did not show variation in the nucleotides of the barcode genes.”
Claim · model-written summary
The authors propose rbcL as a standard barcode for Palmae species.
Verbatim
e0“rbcL has a higher discrimination power (90%) than matK (66.6%) and can thus be potentially used as a standard barcode to discriminate the species of Palmae.”