Abstract
Aphids induce many transcriptional perturbations in their host plants, but the signalling cascades responsible and the effects on plant resistance are largely unknown. Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae The transcription factor WRKY22 is known to be involved in pathogen-triggered immunity, and WRKY22 gene expression has been shown to be induced by aphids. Assessment of aphid population development and feeding behaviour on knockout mutants and overexpression lines showed that WRKY22 increases susceptibility to M. persicae via a mesophyll-located mechanism. mRNA sequencing analysis of aphid-infested wrky22 knockout plants revealed the up-regulation of genes involved in salicylic acid (SA) signalling and down-regulation of genes involved in plant growth and cell-wall loosening. In addition, mechanostimulation of knockout plants by clip cages up-regulated jasmonic acid (JA)-responsive genes, resulting in substantial negative JA-SA crosstalk. Based on this and previous studies, WRKY22 is considered to modulate the interplay between the SA and JA pathways in response to a wide range of biotic and abiotic stimuli. Its induction by aphids and its role in suppressing SA and JA signalling make WRKY22 a potential target for aphids to manipulate host plant defences.
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Claims 7
Claim · model-written summary
WRKY22 increases susceptibility of Arabidopsis to Myzus persicae via a mesophyll-located mechanism.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- knockout mutants and overexpression linese1
- Interactor
- Myzus persicae
Myzus persicaee0pest - Gene
- WRKY22e1
- Outcome
- susceptibility to M. persicaee1
- Direction
- increasese1
- Qualifier
- via a mesophyll-located mechanisme1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e1“Assessment of aphid population development and feeding behaviour on knockout mutants and overexpression lines showed that WRKY22 increases susceptibility to M. persicae via a mesophyll-located mechanism”
Claim · model-written summary
Aphid-infested wrky22 knockout plants showed up-regulation of genes involved in salicylic acid signalling.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- aphid-infestede2
- Interactor
- aphide2pest
- Gene
- wrky22e2
- Outcome
- genes involved in salicylic acid (SA) signallinge2
- Direction
- up-regulatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e2“mRNA sequencing analysis of aphid-infested wrky22 knockout plants revealed the up-regulation of genes involved in salicylic acid (SA) signalling and down-regulation of genes involved in plant growth and cell-wall loosening”
Claim · model-written summary
Aphid-infested wrky22 knockout plants showed down-regulation of genes involved in plant growth and cell-wall loosening.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- aphid-infestede2
- Interactor
- aphide2pest
- Gene
- wrky22e2
- Outcome
- genes involved in plant growth and cell-wall looseninge2
- Direction
- down-regulatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e2“mRNA sequencing analysis of aphid-infested wrky22 knockout plants revealed the up-regulation of genes involved in salicylic acid (SA) signalling and down-regulation of genes involved in plant growth and cell-wall loosening”
Claim · model-written summary
Mechanostimulation of knockout plants by clip cages up-regulated jasmonic acid-responsive genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- clip cagese3
- Outcome
- jasmonic acid (JA)-responsive genese3
- Direction
- up-regulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e3“mechanostimulation of knockout plants by clip cages up-regulated jasmonic acid (JA)-responsive genes, resulting in substantial negative JA-SA crosstalk”
Claim · model-written summary
Clip-cage mechanostimulation of knockout plants produced substantial negative JA-SA crosstalk.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- clip cagese3
- Outcome
- JA-SA crosstalke3
- Direction
- negativee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e3“mechanostimulation of knockout plants by clip cages up-regulated jasmonic acid (JA)-responsive genes, resulting in substantial negative JA-SA crosstalk”
Claim · model-written summary
WRKY22 is considered to modulate the interplay between the SA and JA pathways.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- WRKY22e4
- Outcome
- interplay between the SA and JA pathwayse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e4“WRKY22 is considered to modulate the interplay between the SA and JA pathways in response to a wide range of biotic and abiotic stimuli”
Claim · model-written summary
WRKY22 is a potential target for aphids to manipulate host plant defences.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Interactor
- aphidsAphidomorphae5pest
- Gene
- WRKY22e5
- Outcome
- host plant defencese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae”
e5“Its induction by aphids and its role in suppressing SA and JA signalling make WRKY22 a potential target for aphids to manipulate host plant defences”
The same questions in other papers
overexpression · Myzus persicae · Arabidopsis thaliana (thale cress)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.