Paper · 2010 · Xibei zhiwu xuebao

Genetic polymorphism of starch branching enzyme and its contribution to amylopectin content in wheat grain.

Fa-Mao ZHAO; Qi Xia; Jun Xiao; et al.

5
claims
9
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

The property of starch in wheat(Triticum aestivum L.) grain endosperm is a very important determinant for wheat quality,and it is essential to understand genetic effect of isoforms of starch branching enzyme(SBE) on starch biosynthesis for wheat quality improvement.The starch contents were assessed with 90 wheat cultivars,including 83 domestic hexaploid wheat from different province of China and 7 exotic ones.The genotypes of SBE were also investigated using native polyacrylamide gel electrophoresis(Native-PAGE).The result indicated that there were two loci,SBEⅠ and SBEⅡ,of SBE.SBEⅠ menifested diverse polymorphism with the allele loci of A,B,Dⅰ and Dⅱ.SBEⅡ only exhibited one locus,ie,SBEⅡa.Among all 90 wheat cultivars,seven genotypes were observed based on four allele loci of SBEⅠ.In order to explore the relationship between the genotype and amylopectin content,genetic effects of different genotype on amylopectin content and multiple comparison analysis were conducted.It showed that the amylopectin content of genotypes containing A locus(A DⅰDⅱ and ADⅰB) were relatively higher,which there was significant differences at 0.05 level compared to that of DⅰB and DⅰDⅱ,especially to Dⅰ.It was concluded that there was different genetic effects for different genotype of SBE on amylopectin content.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

Two SBE loci, SBEⅠ and SBEⅡ, were identified in wheat.

Gene
SBEⅠ and SBEⅡe0
Outcome
two locie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“there were two loci,SBEⅠ and SBEⅡ,of SBE”

✓ located verbatim in the source · abstract, characters 544–584 · show in text ↑
e1

“wheat(Triticum aestivum L.) grain endosperm”

✓ located verbatim in the source · abstract, characters 26–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

SBEⅠ showed polymorphism involving A, B, Dⅰ and Dⅱ alleles.

Gene
SBEⅠe0
Outcome
diverse polymorphism with the allele loci of A,B,Dⅰ and Dⅱe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“SBEⅠ menifested diverse polymorphism with the allele loci of A,B,Dⅰ and Dⅱ”

✓ located verbatim in the source · abstract, characters 585–659 · show in text ↑
e1

“wheat(Triticum aestivum L.) grain endosperm”

✓ located verbatim in the source · abstract, characters 26–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

SBEⅡ exhibited only the SBEⅡa locus.

Gene
SBEⅡe0
Outcome
one locus,ie,SBEⅡae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“SBEⅡ only exhibited one locus,ie,SBEⅡa”

✓ located verbatim in the source · abstract, characters 660–698 · show in text ↑
e1

“wheat(Triticum aestivum L.) grain endosperm”

✓ located verbatim in the source · abstract, characters 26–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seven SBEⅠ genotypes were observed among 90 wheat cultivars.

Gene
SBEⅠe0
Outcome
genotypes were observede0
Value
seven genotypese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Among all 90 wheat cultivars,seven genotypes were observed based on four allele loci of SBEⅠ”

✓ located verbatim in the source · abstract, characters 699–791 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Wheat genotypes containing A locus had relatively higher amylopectin content than DⅰB and DⅰDⅱ, particularly Dⅰ, with significant differences at 0.05 level.

Material
genotypes containing A locuse0
Gene
A locuse0
Outcome
amylopectin contente0
Direction
relatively highere0
Qualifier
especially to Dⅰe0
Comparator
DⅰB and DⅰDⅱe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“amylopectin content of genotypes containing A locus(A DⅰDⅱ and ADⅰB) were relatively higher,which there was significant differences at 0.05 level compared to that of DⅰB and DⅰDⅱ,especially to Dⅰ”

✓ located verbatim in the source · abstract, characters 1002–1197 · show in text ↑
e1

“wheat(Triticum aestivum L.) grain endosperm”

✓ located verbatim in the source · abstract, characters 26–69 · show in text ↑