Abstract
The specific primers were designed according to the highly conserved regions of Potato spindle tuber viroid(PSTVd)gene sequence, and were used to detect PSTVd by reverse transcription polymerase chain reaction(RT-PCR).In order to avoid false negative results from RNA degradation or the poor reaction quality of RT-PCR, an internal control which based on the genomic mRNA sequence of potato mitochondrial NADH dehydrogenase ND2 subunit was incorporated in the PSTVd detection protocol. One of the internal con- trol primers was designed and located over the spliced junction of mRNA, the primers were shown to amplify only the spliced RNA derived cDNA but not the genomic DNA itself. Using the internal control primers and the PSTVd primers, two specific PCR fragments were amplified by duplex RT-PCR, which were expected the size of 190 bp for internal control gene and 360 bp for PSTVd, respectively. The internal control could effec- tively inspect the detection process of PSTVd by duplex RT-PCR.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Internal control primers amplified spliced RNA derived cDNA but not genomic DNA.
- Taxon
- potato
Solanum tuberosume1 - Gene
- potato mitochondrial NADH dehydrogenase ND2 subunite1
- Outcome
- spliced RNA derived cDNAe0
- Comparator
- genomic DNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the primers were shown to amplify only the spliced RNA derived cDNA but not the genomic DNA itself.”
e1“potato mitochondrial NADH dehydrogenase ND2 subunit”
Claim · model-written summary
Duplex RT-PCR amplified a 190 bp internal control fragment.
- Taxon
- potatoSolanum tuberosume1
- Outcome
- internal control genee0
- Value
- 190 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two specific PCR fragments were amplified by duplex RT-PCR, which were expected the size of 190 bp for internal control gene and 360 bp for PSTVd, respectively.”
e1“potato mitochondrial NADH dehydrogenase ND2 subunit”
Claim · model-written summary
Duplex RT-PCR amplified a 360 bp PSTVd fragment.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- PSTVde0pathogen
- Outcome
- PSTVde0
- Value
- 360 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two specific PCR fragments were amplified by duplex RT-PCR, which were expected the size of 190 bp for internal control gene and 360 bp for PSTVd, respectively.”
e1“potato mitochondrial NADH dehydrogenase ND2 subunit”
Claim · model-written summary
The internal control effectively checked the duplex RT-PCR detection process for PSTVd.
- Taxon
- potatoSolanum tuberosume1
- Interactor
- PSTVde0pathogen
- Outcome
- detection process of PSTVde0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The internal control could effec- tively inspect the detection process of PSTVd by duplex RT-PCR.”
e1“potato mitochondrial NADH dehydrogenase ND2 subunit”