Abstract
The microRNAs(miRNAs) are a newly identified class of small non-coding RNA,which regulate post-transcriptional gene expressing in eukaryote.Most miRNAs are predicted through the computer,the presentence/absence and the spatial/temporal of miRNAs still unclear.It is important for the establishment of efficient,sensitive and specific detection methods to detect miRNAs in different organism or period.In this study,the protocol derived from Lau et al.and Shi et al.was used to detect miR156a,predicted and released by Sanger Institute,in old or young leaf,root,and shoot for verifying this method.By comparison,this method is low cost,simple,fast,and sensitive,common for primer of RT-PCR,which could identify a number of miRNAs in the same organism and compare miRNAs from different organism.Furthermore,this method need less RNA,in comparison with Northern Blot and Microarray.
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Claims 3
Claim · model-written summary
The RT-PCR method was low cost, simple, fast and sensitive.
- Taxon
- Soybean
Glycine maxt0 - Outcome
- low cost,simple,fast,and sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this method is low cost,simple,fast,and sensitive”
t0“Soybean”
Claim · model-written summary
The method could identify multiple miRNAs in one organism and compare miRNAs across organisms.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- identify a number of miRNAs in the same organism and compare miRNAs from different organisme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could identify a number of miRNAs in the same organism and compare miRNAs from different organism”
t0“Soybean”
Claim · model-written summary
The method required less RNA than Northern Blot and Microarray.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- RNAe0
- Direction
- lesse0
- Comparator
- Northern Blot and Microarraye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this method need less RNA,in comparison with Northern Blot and Microarray”
t0“Soybean”