Abstract
Accurate identification of varieties (clones) and understanding their genetic relationships are essential for the poplar breeding and variety management.In this study,21 elite poplar varieties of Tacamahaca and Aigeiros were fingerprinted using AFLP markers.Four AFLP-primer pairs developed generated totally 181 AFLP polymorphic fragments,and in particular,each primer pair generated fingerprint profiles specific to each of the tested varieties.The genetic relationships among the varieties were evaluated by dendrograms and Multidimensional Scaling (MDS),showing that the tested poplar can be classified into five groups.The results also indicated the clear-cup separation of varieties of different sections of poplar and the primary distinction between native and exotic poplar varieties.Present study indicated that tested poplar varieties could be identified by their fingerprint profiles and that genetic relationships deducted from the varieties study were consistent with their genealogy.In addition,our results demonstrated that AFLP could be used to construct DNA fingerprints of poplar clones at large-scale level and to determine genetic relationships of poplar varieties.
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Claims 7
Claim · model-written summary
Four AFLP primer pairs produced 181 polymorphic fragments.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four AFLP-primer pairs developed generated totally 181 AFLP polymorphic fragments”
t0“Genus Populus”
Claim · model-written summary
Each primer pair yielded fingerprints specific to the tested varieties.
- Outcome
- fingerprint profiles specific to each of the tested varietiese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each primer pair generated fingerprint profiles specific to each of the tested varieties”
t0“Genus Populus”
Claim · model-written summary
The tested poplars were classified into five groups.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the tested poplar can be classified into five groups”
Claim · model-written summary
The analysis separated varieties from different sections.
- Outcome
- separation of varieties of different sectionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the clear-cup separation of varieties of different sections of poplar”
Claim · model-written summary
The analysis distinguished native and exotic varieties.
- Outcome
- distinction between native and exotic poplar varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the primary distinction between native and exotic poplar varieties”
Claim · model-written summary
The inferred genetic relationships agreed with genealogy.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic relationships deducted from the varieties study were consistent with their genealogy”
t0“Genus Populus”
Claim · model-written summary
AFLP was judged suitable for large-scale clone fingerprints and genetic relationships.
- Outcome
- DNA fingerprints of poplar clones at large-scale levele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AFLP could be used to construct DNA fingerprints of poplar clones at large-scale level and to determine genetic relationships of poplar varieties”