Abstract
The seeds of three indica rice(Oryza sativa L.subsp.indica) Qiuguiai11、Huaxianzhan and Wanxianzhan 45 were collected.Those were carried out on different basal media after sterilization treatment,several media with different combination of growth regulators were tried.After 20～30 days of incubation under dark at 25±1℃,calli initiated from Scutellum were subcultured on fresh callus induction media.After 20 days,Systemic researches had been carried for callus inducing ratio and the maximum proliferation efficiency of embryogenic callus.High frequency induction from mature seeds derived embryogenic callus was initiated on NMB combination medium supplement with 2.5～3.0 mg/L 2,4-D,for nutrient substance such as proline,casein acid hydrolysate and D-sorbitol.it is beneficial only for Wanxianzhan45.Those calli were sub cultured in multiplied media.in NPCS medium(basal medium NMB+0.5 g/L proline+0.3 g/L CH+3～5 g/L D-sorbitol) with 30 g/L maltose and 2.5 mg/L 2,4-D,the multiplied vigorously callus and the maximum proliferation of embryogenic callus were obtained.
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Claims 3
Claim · model-written summary
High-frequency embryogenic callus induction from mature seeds on NMB medium with 2.5 to 3.0 mg/L 2,4-D was beneficial only for Wanxianzhan45
- Taxon
- Oryza sativa
Oryza sativat0 - Material
- Wanxianzhan45e2
- Organ / stage
- mature seedse1
- Treatment
- 2.5～3.0 mg/L 2,4-De1
- Outcome
- High frequency inductione1
- Direction
- beneficiale2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice(Oryza sativa L.subsp.indica)”
e1“High frequency induction from mature seeds derived embryogenic callus was initiated on NMB combination medium supplement with 2.5～3.0 mg/L 2,4-D”
e2“it is beneficial only for Wanxianzhan45”
Claim · model-written summary
NPCS medium with 30 g/L maltose and 2.5 mg/L 2,4-D gave the maximum proliferation of embryogenic callus
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- 30 g/L maltose and 2.5 mg/L 2,4-De3
- Outcome
- maximum proliferation of embryogenic calluse3
- Direction
- maximume3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice(Oryza sativa L.subsp.indica)”
e3“with 30 g/L maltose and 2.5 mg/L 2,4-D, the multiplied vigorously callus and the maximum proliferation of embryogenic callus were obtained”
Claim · model-written summary
The same NPCS medium gave vigorously multiplied callus
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- 30 g/L maltose and 2.5 mg/L 2,4-De3
- Outcome
- multiplied vigorously calluse3
- Direction
- multiplied vigorouslye3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice(Oryza sativa L.subsp.indica)”
e3“with 30 g/L maltose and 2.5 mg/L 2,4-D, the multiplied vigorously callus and the maximum proliferation of embryogenic callus were obtained”
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2,4-D · growth · Oryza sativa (rice)Positive and negative results
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- negative
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.