Abstract
To screen soybean lines resistant to glyphosate through chemomorphosis,six soybean varieties widly planted in Heilongjiang Province were dealt with chemical mutagen ethyl methane sulphonate(EMS)and sodium azide by seed soaking.The survival rate of soybean seeds in M1 generation was higher than that of semi-lethal concentrations.In M3 generation,35518 plants were identified by spraying 1.31ai.kg·ha-1 glyphosate at three-leaf stage,from which a total of 40 resistant plants showed normal growth,and they were Suinong 10 and Heinong 44 dealt with 5 mmol·L-1 sodium azide.The M4 generation of the 40 survival lines also showed glyphosate resistance.Results suggest chemical mutagen could be used in selecting soybean mutant lines resistant to glyphosate.
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Claims 5
Claim · model-written summary
M1 soybean seed survival exceeded that at semi-lethal concentrations.
- Taxon
- soybean
Glycine maxe0t0 - Outcome
- survival ratee0
- Direction
- highere0
- Comparator
- semi-lethal concentrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The survival rate of soybean seeds in M1 generation was higher than that of semi-lethal concentrations”
t0“Soybean”
Claim · model-written summary
Forty M3 plants among 35,518 screened grew normally after glyphosate spray.
- Taxon
- plantsViridiplantaee0
- Treatment
- 1.31ai.kg·ha-1 glyphosatee0
- Outcome
- resistant plantse0
- Value
- 40e0
Not reportedComparatorDirection
Verbatim
e0“In M3 generation,35518 plants were identified by spraying 1.31ai.kg·ha-1 glyphosate at three-leaf stage,from which a total of 40 resistant plants showed normal growth”
Claim · model-written summary
The resistant plants were sodium azide treated Suinong 10 and Heinong 44.
- Taxon
- soybeanGlycine maxe0t0
- Material
- Suinong 10 and Heinong 44e0
- Treatment
- 5 mmol·L-1 sodium azidee0
- Outcome
- Suinong 10 and Heinong 44e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were Suinong 10 and Heinong 44 dealt with 5 mmol·L-1 sodium azide”
t0“Soybean”
Claim · model-written summary
M4 progeny of the 40 surviving lines remained glyphosate resistant.
- Taxon
- soybeanGlycine maxe0t0
- Material
- 40 survival linese0
- Outcome
- glyphosate resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The M4 generation of the 40 survival lines also showed glyphosate resistance”
t0“Soybean”
Claim · model-written summary
The authors suggested chemical mutagens can select glyphosate resistant soybean lines.
- Taxon
- soybeanGlycine maxe0t0
- Outcome
- selecting soybean mutant lines resistant to glyphosatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results suggest chemical mutagen could be used in selecting soybean mutant lines resistant to glyphosate”
t0“Soybean”