Paper · 2008 · Acta Agriculturae Boreali-Sinica

Analysis of DNA Methylation Related on the Interaction between Wheat and Wheat Leaf Rust

Daqun Liu

4
claims
6
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes.The wheat near-isogenic line TcLr19 and Thatcher is race-specific resistance and compatible in the interaction with Puccinia triticina respectively.Using methylation-sensitive amplified polymorphism(MSAP) analysis,we compared the patterns of cytosine methylation in TcLr19 and Thatcher that had been inoculated with Puccinia triticina,subjected to left untreated.In all,3 554 DNA fragments,each representing a recognition site cleaved by either or both of two isoschizomers,were amplified using 60 pairs of selective primers.A total of 998 sites were found to be one of the methylated.The results suggest that no differences in cytosine methylation exist between seedlings inoculated and those unchallenged.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

The wheat line TcLr19 was resistant to Puccinia triticina.

Material
TcLr19e0
Interactor
Puccinia triticinaPuccinia triticinae0pathogen
Outcome
race-specific resistancee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Puccinia triticina
Puccinia triticina · InteractorRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The wheat near-isogenic line TcLr19 and Thatcher is race-specific resistance and compatible in the interaction with Puccinia triticina respectively.”

✓ located verbatim in the source · abstract, characters 102–250 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The wheat line Thatcher was compatible with Puccinia triticina.

Material
Thatchere0
Interactor
Puccinia triticinaPuccinia triticinae0pathogen
Outcome
compatiblee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Puccinia triticina
Puccinia triticina · InteractorRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The wheat near-isogenic line TcLr19 and Thatcher is race-specific resistance and compatible in the interaction with Puccinia triticina respectively.”

✓ located verbatim in the source · abstract, characters 102–250 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A total of 998 assayed wheat sites were methylated.

Outcome
methylatede0
Value
998 sitese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“A total of 998 sites were found to be one of the methylated.”

✓ located verbatim in the source · abstract, characters 627–687 · show in text ↑
t0

“Wheat and Wheat Leaf Rust”

✓ located verbatim in the source · title, characters 63–88 · show in text ↑
Intervention effectauthor interpretationoriginal resultno effectmeasured

Claim · model-written summary

Cytosine methylation did not differ between inoculated and unchallenged wheat seedlings.

Treatment
inoculatede0
Outcome
cytosine methylatione0
Direction
no differencese0
Comparator
unchallengede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The results suggest that no differences in cytosine methylation exist between seedlings inoculated and those unchallenged.”

✓ located verbatim in the source · abstract, characters 687–809 · show in text ↑
t0

“Wheat and Wheat Leaf Rust”

✓ located verbatim in the source · title, characters 63–88 · show in text ↑

inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results

47 papers: 38 positive, 9 negative or no effect

8 papers in the same direction as this paper · 38 in the opposite direction

  1. positive
  2. positive
  3. positive

43 papers more in this group

See all 47 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.