Abstract
【Objective】In this study,resistance gene homology fragments from wheat were isolated using homology-based method.【Method】Two pairs of primers were designed according to the amino acid conserved regions NBS-LRR of the cloned plant disease resistance genes,and the reverse transcription-polymerase chain reaction(RT-PCR) was used.【Result】Three open-reading NBS resistance gene analogues(RGAs) have been obtained.They were named as PS13-1,PS13-2,and S2A2 respectively from the RNA of TcLr35 carrying the Lr35 gene conferring resistance against wheat leaf rust by a reverse transcription-polymerase chain reaction(RT-PCR).The nucleotide sequences of the three RGAs were 239bp,289bp and 539bp encoding 78,84 and 177 amino acids respectively.Homology research showed that the nucleotides of PS13-1 and PS13-2 were 91% identical to wheat powdery mildew resistance gene PM3b,and the nucleotide of S2A2 was 91% identical to the sequence from the mRNA of barley.BLASTp analysis indicated that three RGAs contained the conserved motifs of NB-ARC(such as P-loop,kinase2,kinase3a and transmembrance domain).Northern hybridization showed that the RGAs expression was not induced by Puccinia recondite inoculation suggesting that the gene be a constitutive gene with low abundance in wheat.【Conclusion】In this study,three resistance homology sequence were obtained in TcLr35,which provide the shortcut for the cloning of wheat leaf rust resistance genes.
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Claims 10
Claim · model-written summary
Three open-reading NBS resistance gene analogues were obtained from wheat TcLr35 RNA.
- Taxon
- wheat
Triticum aestivume0 - Material
- TcLr35e0
- Gene
- PS13-1,PS13-2,and S2A2e0
- Outcome
- Three open-reading NBS resistance gene analogues(RGAs)e0
- Value
- Threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three open-reading NBS resistance gene analogues(RGAs) have been obtained.They were named as PS13-1,PS13-2,and S2A2 respectively from the RNA of TcLr35 carrying the Lr35 gene conferring resistance against wheat leaf rust”
Claim · model-written summary
PS13-1 had a 239bp nucleotide sequence encoding 78 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide sequences of the three RGAs were 239bp,289bp and 539bp encoding 78,84 and 177 amino acids respectively.”
e1“They were named as PS13-1,PS13-2,and S2A2 respectively”
t0“Wheat”
Claim · model-written summary
PS13-2 had a 289bp nucleotide sequence encoding 84 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide sequences of the three RGAs were 239bp,289bp and 539bp encoding 78,84 and 177 amino acids respectively.”
e1“They were named as PS13-1,PS13-2,and S2A2 respectively”
t0“Wheat”
Claim · model-written summary
S2A2 had a 539bp nucleotide sequence encoding 177 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nucleotide sequences of the three RGAs were 239bp,289bp and 539bp encoding 78,84 and 177 amino acids respectively.”
e1“They were named as PS13-1,PS13-2,and S2A2 respectively”
t0“Wheat”
Claim · model-written summary
PS13-1 nucleotides were 91% identical to wheat PM3b.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nucleotides of PS13-1 and PS13-2 were 91% identical to wheat powdery mildew resistance gene PM3b”
Claim · model-written summary
PS13-2 nucleotides were 91% identical to wheat PM3b.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nucleotides of PS13-1 and PS13-2 were 91% identical to wheat powdery mildew resistance gene PM3b”
Claim · model-written summary
S2A2 nucleotides were 91% identical to a barley mRNA sequence.
- Taxon
- WheatTriticum aestivumt0
- Gene
- S2A2e0
- Outcome
- identicale0
- Value
- 91%e0
- Comparator
- sequence from the mRNA of barleye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nucleotide of S2A2 was 91% identical to the sequence from the mRNA of barley”
t0“Wheat”
Claim · model-written summary
Three wheat RGAs contained conserved NB-ARC motifs including P-loop, kinase2, kinase3a and transmembrance domain.
- Taxon
- WheatTriticum aestivumt0
- Gene
- NB-ARCe0
- Outcome
- conserved motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three RGAs contained the conserved motifs of NB-ARC(such as P-loop,kinase2,kinase3a and transmembrance domain)”
t0“Wheat”
Claim · model-written summary
Puccinia recondite inoculation did not induce expression of wheat RGAs.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Puccinia recondite inoculatione0
- Interactor
- Puccinia reconditee0pathogen
- Gene
- RGAse0
- Outcome
- RGAs expressione0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Northern hybridization showed that the RGAs expression was not induced by Puccinia recondite inoculation suggesting that the gene be a constitutive gene with low abundance in wheat.”
Claim · model-written summary
The wheat RGA was interpreted as constitutively expressed at low abundance.
- Taxon
- wheatTriticum aestivume0
- Gene
- genee0
- Outcome
- constitutive gene with low abundancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that the gene be a constitutive gene with low abundance in wheat”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
8 papers in the same direction as this paper · 38 in the opposite direction
- positive
- positive
- positive
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