Abstract
Objective:In order to further identify the differences in three representative samples of Gastrodia elata,their ITS sequences of rDNA genes were amplified,sequenced and analyzed.It is indicated that there are different relationship between them from the level of molecular variation.Method:Genomic DNA from Gastrodia elata extracted by modified CTAB method were regarded as templates for PCR amplification,cloning and sequencing by synthetic specific primer the nrDNA ITS sequences.Result:G.elata Bl.f.flavida S.Chow and G.elata Bl.f.viridis Mak constitute the first two sister groups,their genetic distance were about 25,G.elata Bl.f.glauca S.Chow and their genetic distance were about 50 and the genetic distance between them has reached the 100% confidence.In the amplified ITS1 sequence with 209bp,there are 15 varied base sites which could be used as specific characters to identify three types.In terms of the natural population scale,G.elata Bl.f.glauca is much richer than other types in Genetic diversity,it might be the original type of G.elata species in phylogeny and evolution.Conclusion: From the above,sequence analysis of ITS provides a new approach to identify the Gastrodia elata resources.
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Claims 7
Claim · model-written summary
G.elata f. flavida and G.elata f. viridis are the first two sister groups, with a genetic distance of about 25.
- Taxon
- G.elata Bl.f.flavida S.Chow
Gastrodia elatae1 - Outcome
- genetic distancee1
- Value
- about 25e1
- Qualifier
- constitute the first two sister groupse1
- Comparator
- G.elata Bl.f.viridis Make1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“G.elata Bl.f.flavida S.Chow and G.elata Bl.f.viridis Mak constitute the first two sister groups,their genetic distance were about 25”
Claim · model-written summary
G.elata f. glauca has a genetic distance of about 50 from the other types.
- Taxon
- G.elata Bl.f.glauca S.ChowGastrodia elatae2
- Outcome
- genetic distancee2
- Value
- about 50e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“G.elata Bl.f.glauca S.Chow and their genetic distance were about 50”
Claim · model-written summary
The genetic distance between the types was supported at 100% confidence.
- Taxon
- Gastrodia elataGastrodia elatat0
- Outcome
- genetic distance between theme3
- Value
- 100% confidencee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of Different Types in Gastrodia elata Based on the Analysis of ITS Sequence”
e3“the genetic distance between them has reached the 100% confidence”
Claim · model-written summary
The ITS1 sequence contains 15 varied base sites that can serve as specific characters to identify the three types.
- Taxon
- Gastrodia elataGastrodia elatat0
- Outcome
- varied base sitese4
- Value
- 15e4
- Qualifier
- In the amplified ITS1 sequence with 209bpe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Identification of Different Types in Gastrodia elata Based on the Analysis of ITS Sequence”
e4“there are 15 varied base sites which could be used as specific characters to identify three types”
e5“In the amplified ITS1 sequence with 209bp”
Claim · model-written summary
G.elata f. glauca is richer in genetic diversity than the other types in natural populations.
- Taxon
- G.elata Bl.f.glaucaGastrodia elatae6
- Outcome
- Genetic diversitye6
- Direction
- richere6
- Qualifier
- In terms of the natural population scalee7
- Comparator
- other typese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“G.elata Bl.f.glauca is much richer than other types in Genetic diversity”
e7“In terms of the natural population scale”
Claim · model-written summary
G.elata f. glauca might be the original type of the species in phylogeny and evolution.
- Taxon
- G.elataGastrodia elatae0
- Outcome
- original type of G.elata speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it might be the original type of G.elata species in phylogeny and evolution”
Claim · model-written summary
ITS sequence analysis provides a new approach to identify Gastrodia elata resources.
- Taxon
- Gastrodia elataGastrodia elatae1
- Outcome
- new approach to identify the Gastrodia elata resourcese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“sequence analysis of ITS provides a new approach to identify the Gastrodia elata resources”