Abstract
OBJECTIVE To investigate the genetic diversity of yam resources in China and provide reliable molecular evidences for cultivar identification and genetic relationship.METHODS Sequence-related amplified polymorphism(SRAP) was applied to detect the genetic diversity of 21 yam cultivars from eight cultivated populations,Popgene software(version 1.32) was used to calculate genetic parameters,UPGMA clustering was carried out using Mega software(version 4.1),and principal component analysis was performed with the help of Ntsys-pc(Version 2.1) program.RESULTS Three hundreds and nine bands were amplified by 20 SRAP primer combinations,of which 289 bands were polymorphic.The Nei′s gene diversity index(H=0.288 1),Shannon′s information index(I=0.441 6) and total genetic diversity(Ht=0.289 1) revealed a relatively high genetic diversity level among yam populations.Within populations,HNWX population showed the highest genetic diversity(PPB= 35.92%),whereas the lowest diversity(PPB=0) was observed in FJSM and ZJRG populations.The genetic differentiation coefficient(Gst=0.865 8) and genetic flow(Nm=0.007 5) indicated that the genetic diversity among populations was higher than that of within populations.A well-separated groups coupling with cultivated species was formed according to the genetic distance(GD) ranging from 0.049 8-0.487 9 of the investigated populations.Twenty-one investigated cultivars could be distinguished and be effectively grouped into four origins,i.e.,Dioscorea opposita Thunb.,Dioscorea alata Linn.,Dioscorea persimilis Prain et Burkill.and Dioscorea fordii Prain et Burkill..CONCLUSION The genetic diversity level of yam resources is high,and relatively high genetic variation exists among four yam species.SRAP marker is an effective method to identify and classify numerous yam cultivars.
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Claims 11
Claim · model-written summary
SRAP primers produced 309 bands, 289 polymorphic.
Verbatim
e0“Three hundreds and nine bands were amplified by 20 SRAP primer combinations,of which 289 bands were polymorphic.”
t0“Yam”
Claim · model-written summary
Yam populations had relatively high genetic diversity, with Nei index 0.288 1.
Verbatim
e0“Nei′s gene diversity index(H=0.288 1),Shannon′s information index(I=0.441 6) and total genetic diversity(Ht=0.289 1) revealed a relatively high genetic diversity level among yam populations.”
t0“Yam”
Claim · model-written summary
Shannon information index was 0.441 6.
Verbatim
e0“Shannon′s information index(I=0.441 6)”
t0“Yam”
Claim · model-written summary
Total genetic diversity was 0.289 1.
Verbatim
e0“total genetic diversity(Ht=0.289 1)”
t0“Yam”
Claim · model-written summary
HNWX had the highest within-population diversity, 35.92%.
Verbatim
e0“HNWX population showed the highest genetic diversity(PPB= 35.92%)”
t0“Yam”
Claim · model-written summary
FJSM and ZJRG had the lowest within-population diversity, zero.
Verbatim
e0“lowest diversity(PPB=0) was observed in FJSM and ZJRG populations”
t0“Yam”
Claim · model-written summary
Genetic differentiation coefficient was 0.865 8.
Verbatim
e0“genetic differentiation coefficient(Gst=0.865 8)”
t0“Yam”
Claim · model-written summary
Gene flow was 0.007 5.
Verbatim
e0“genetic flow(Nm=0.007 5)”
t0“Yam”
Claim · model-written summary
Genetic diversity among populations exceeded diversity within populations.
Verbatim
e0“genetic diversity among populations was higher than that of within populations”
t0“Yam”
Claim · model-written summary
Genetic distances ranged from 0.049 8-0.487 9 and groups matched cultivated species.
Verbatim
e0“A well-separated groups coupling with cultivated species was formed according to the genetic distance(GD) ranging from 0.049 8-0.487 9 of the investigated populations.”
t0“Yam”
Claim · model-written summary
Twenty-one yam cultivars were distinguished into four species origins.
Verbatim
e0“Twenty-one investigated cultivars could be distinguished and be effectively grouped into four origins,i.e.,Dioscorea opposita Thunb.,Dioscorea alata Linn.,Dioscorea persimilis Prain et Burkill.and Dioscorea fordii Prain et Burkill.”
t0“Yam”