Abstract
The CaMV35S promoter driving plant expression vector pAM22 with Sesbania rostrata phytochelatin synthase 1(SrPCS1) was constructed using PCR and ligation based on the pHANNIBAL and pART27.By electroporation transformation,pAM22 was transferred into Agrobacterium tumefacien EHA105 and the new engineering bacterium was transferred into Nicotiana tabacum.The expression level of SrPCS1 was indentified by antibiotics screening,PCR and northern-blot analysis.At the same time,phylogenetic analysis of SrPCS1 with other PCSs was conducted by ClustalW.The results indicated that SrPCS1 encoding 233 amino-acids exhibited high homologous with PCS from legumes,27 transgenic tobacco tolerant to antibiotics have been acquired in which 23 are positive plants identified by PCR,and 14 transgenic tobacco expressed SrPCS1 plaqnts were identified with northern-blotting analysis,but their expression levels are different.
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Claims 5
Claim · model-written summary
SrPCS1 exhibited high homology with phytochelatin synthases from legumes.
- Taxon
- Sesbania rostrataSesbania rostratat0
- Gene
- SrPCS1e0
- Outcome
- homologous with PCS from legumese0
- Direction
- highe0
Verbatim
e0“SrPCS1 encoding 233 amino-acids exhibited high homologous with PCS from legumes”
t0“Sesbania rostrata Phytochelatin Synthase 1 Gene”
Claim · model-written summary
27 transgenic tobacco plants tolerant to antibiotics were obtained.
- Taxon
- Nicotiana tabacum
Nicotiana tabacumt1 - Outcome
- tolerant to antibioticse0
- Value
- 27e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“27 transgenic tobacco tolerant to antibiotics have been acquired”
t1“Transgenic Nicotiana tabacum”
Claim · model-written summary
23 of the transgenic tobacco plants were positive by PCR.
- Taxon
- Nicotiana tabacumNicotiana tabacumt1
- Outcome
- positive plants identified by PCRe0
- Value
- 23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in which 23 are positive plants identified by PCR”
t1“Transgenic Nicotiana tabacum”
Claim · model-written summary
14 transgenic tobacco plants expressed SrPCS1 according to northern-blot analysis.
- Taxon
- Nicotiana tabacumNicotiana tabacumt1
- Gene
- SrPCS1e0
- Outcome
- expressed SrPCS1e0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14 transgenic tobacco expressed SrPCS1 plaqnts were identified with northern-blotting analysis”
t1“Transgenic Nicotiana tabacum”
Claim · model-written summary
The SrPCS1 expression levels differed among the transgenic tobacco plants.
- Taxon
- Nicotiana tabacumNicotiana tabacumt1
- Outcome
- expression levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but their expression levels are different”
t1“Transgenic Nicotiana tabacum”