Abstract
The regulatory effects of different hormones and their combinations on organogenesis of the segments fromthe main inflorescence stalk of rape,and on the regeneration potentialitiesamong the segments with various dis-tances from apex and kinds of tissueswere examined.The results showedthat BA (2 mg/l) added alone greatlystimulated bud formation.Adding NAA(0.05 or 0.5mg/l) to the BA me-dium markedly decreased both the per-centage of the explants forming budsand the number of buds on each explantThe effect of B_5 medium was superiorfor bud formation than MS medium.Comparative experiments with segmentsfrom different regions of the inflore-scence stalk showed that the youngsubapical part of stalk possessed thehighest capacity of bud formation.The rind tissues (consisting of epider-mis,cortex and vascular tissues) peeledoff from the stalk and the pith tissueswere cultured separately.In this wayit caused a notable decrease of budinitiation,but it showed very littleeffect on both rhizogenesis and callusformtion.It seemed that the strong regenerating capacity of rape inflorescence stalk segments may offer a new technique for the maintaining andrapid propagating of some breedingmaterials,particularly,male-sterile str-ains.
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Claims 8
Claim · model-written summary
BA at 2 mg/l added alone greatly stimulated bud formation in rape stalk explants.
- Taxon
- BRASSICA NAPUS
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Organ / stage
- INFLORESCENCE STALK EXPLANTSt0
- Treatment
- BA (2 mg/l) added alonee1
- Outcome
- bud formatione1
- Direction
- greatly stimulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e1“BA (2 mg/l) added alone greatly stimulated bud formation”
Claim · model-written summary
Adding NAA to BA medium decreased the percentage of explants forming buds.
- Taxon
- BRASSICA NAPUSBrassica napust0
- Treatment
- NAA(0.05 or 0.5mg/l)e2
- Outcome
- explants forming budse2
- Direction
- markedly decreasede2
- Comparator
- BA me-diume2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e2“Adding NAA(0.05 or 0.5mg/l) to the BA me-dium markedly decreased both the per-centage of the explants forming budsand the number of buds on each explant”
Claim · model-written summary
Adding NAA to BA medium decreased the number of buds on each explant.
- Taxon
- BRASSICA NAPUSBrassica napust0
- Treatment
- NAA(0.05 or 0.5mg/l)e2
- Outcome
- the number of buds on each explante2
- Direction
- markedly decreasede2
- Comparator
- BA me-diume2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e2“Adding NAA(0.05 or 0.5mg/l) to the BA me-dium markedly decreased both the per-centage of the explants forming budsand the number of buds on each explant”
Claim · model-written summary
B5 medium was superior to MS medium for bud formation.
- Taxon
- BRASSICA NAPUSBrassica napust0
- Treatment
- B_5 mediume3
- Outcome
- bud formatione3
- Direction
- superiore3
- Comparator
- MS mediume3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e3“The effect of B_5 medium was superiorfor bud formation than MS medium”
Claim · model-written summary
Removing the rind tissues caused a notable decrease in bud initiation.
- Taxon
- BRASSICA NAPUSBrassica napust0
- Treatment
- rind tissuese6
- Outcome
- budinitiatione5
- Direction
- decreasee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e6“The rind tissues”
e5“caused a notable decrease of budinitiation”
Claim · model-written summary
Rind tissues had very little effect on rhizogenesis.
- Taxon
- BRASSICA NAPUSBrassica napust0
- Treatment
- rind tissuese6
- Outcome
- rhizogenesise7
- Direction
- very littleeffecte7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e6“The rind tissues”
e7“showed very littleeffect on both rhizogenesis and callusformtion”
Claim · model-written summary
Rind tissues had very little effect on callus formation.
- Taxon
- BRASSICA NAPUSBrassica napust0
- Treatment
- rind tissuese6
- Outcome
- callusformtione7
- Direction
- very littleeffecte7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e6“The rind tissues”
e7“showed very littleeffect on both rhizogenesis and callusformtion”
Claim · model-written summary
Stalk segments with strong regenerating capacity may offer a new technique for rapid propagating of breeding materials, such as male-sterile strains.
- Taxon
- rapeBrassica napust0
- Material
- male-sterile str-ainse0
- Outcome
- rapid propagatinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“INFLORESCENCE STALK EXPLANTS OF RAPE (BRASSICA NAPUS L.)”
e0“may offer a new technique for the maintaining andrapid propagating of some breedingmaterials,particularly,male-sterile str-ains”