Paper · 2011 · Guoshu xuebao

Cloning and transcriptional analysis of resistance gene Fom-2 from Cucumis melo ssp.Agrestis Jeffrey

Mingfang Zhang

6
claims
12
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

Fusarium wilt(Fusarium oxysporum f.sp.melonis Snyder and Hansen) is one of destructive disease in melon crop production.Fom-2 gene was genetically identified to control the resistance of races 0 and 1.Resistance to fusarium wilt breeding is one of important targets for melon breeding.In this study,we cloned the Fom-2 gene from Baizhuogua melon(Cucumis melo ssp.agrestis Jeffrey) by using homological cloning method,which was deduced to encode 1099 amino acids and exhibited high similarity to NBS-LRR type of R genes in plant.The deduced FOM-2 protein contained NB-ARC and SCOP typical motifs of R gene.The expression of Fom-2 gene was highly induced after inoculation with fom race 1.In conclusion,the cloning of Fom-2 gene is good for breeding with resistance to fusarium wilt.

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Claims 6

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Fom-2 was cloned from Baizhuogua melon.

Taxon
melonCucumis meloe0
Material
Baizhuoguae0
Gene
Fom-2e0
Outcome
cloned the Fom-2 genee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis melo
Cucumis melo · TaxonUSDA photo by Scott Bauer. Image Number K7355-11. · Public domain · Wikimedia Commons ↗

Verbatim

e0

“we cloned the Fom-2 gene from Baizhuogua melon(Cucumis melo ssp.agrestis Jeffrey)”

✓ located verbatim in the source · abstract, characters 299–380 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Fom-2 was predicted to encode 1099 amino acids.

Taxon
melonCucumis meloe1
Gene
Fom-2t0
Outcome
encode 1099 amino acidse0
Value
1099 amino acidse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis melo
Cucumis melo · TaxonUSDA photo by Scott Bauer. Image Number K7355-11. · Public domain · Wikimedia Commons ↗

Verbatim

e0

“which was deduced to encode 1099 amino acids”

✓ located verbatim in the source · abstract, characters 417–461 · show in text ↑
t0

“resistance gene Fom-2 from Cucumis melo”

✓ located verbatim in the source · title, characters 40–79 · show in text ↑
e1

“Baizhuogua melon”

✓ located verbatim in the source · abstract, characters 329–345 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Fom-2 was highly similar to NBS-LRR resistance genes.

Taxon
Cucumis meloCucumis melot0
Gene
Fom-2t0
Outcome
high similarity to NBS-LRR type of R genese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis melo
Cucumis melo · TaxonUSDA photo by Scott Bauer. Image Number K7355-11. · Public domain · Wikimedia Commons ↗

Verbatim

e0

“exhibited high similarity to NBS-LRR type of R genes in plant”

✓ located verbatim in the source · abstract, characters 466–527 · show in text ↑
t0

“resistance gene Fom-2 from Cucumis melo”

✓ located verbatim in the source · title, characters 40–79 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

FOM-2 contained NB-ARC and SCOP motifs.

Taxon
Cucumis meloCucumis melot0
Gene
FOM-2e0
Outcome
contained NB-ARC and SCOP typical motifse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis melo
Cucumis melo · TaxonUSDA photo by Scott Bauer. Image Number K7355-11. · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The deduced FOM-2 protein contained NB-ARC and SCOP typical motifs of R gene.”

✓ located verbatim in the source · abstract, characters 528–605 · show in text ↑
t0

“Cucumis melo”

✓ located verbatim in the source · title, characters 67–79 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fom-2 expression was highly induced after inoculation with fom race 1.

Taxon
Cucumis meloCucumis melot0
Treatment
inoculation with fom race 1e0
Interactor
fom race 1e0pathogen
Gene
Fom-2e0
Outcome
expression of Fom-2 genee0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis melo
Cucumis melo · TaxonUSDA photo by Scott Bauer. Image Number K7355-11. · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The expression of Fom-2 gene was highly induced after inoculation with fom race 1.”

✓ located verbatim in the source · abstract, characters 605–687 · show in text ↑
t0

“Cucumis melo”

✓ located verbatim in the source · title, characters 67–79 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors conclude that Fom-2 cloning supports Fusarium wilt resistance breeding.

Taxon
melonCucumis meloe1
Gene
Fom-2e0
Outcome
breeding with resistance to fusarium wilte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis melo
Cucumis melo · TaxonUSDA photo by Scott Bauer. Image Number K7355-11. · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the cloning of Fom-2 gene is good for breeding with resistance to fusarium wilt”

✓ located verbatim in the source · abstract, characters 701–780 · show in text ↑
e1

“melon crop production”

✓ located verbatim in the source · abstract, characters 98–119 · show in text ↑

inoculation · molecular: expression · Cucumis melo (muskmelon)Results agree

3 papers: 3 positive, 0 negative or no effect

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  2. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.