Abstract
In order to breed new waxy spring wheat lines that adapt to the environment of Qinghai Plateau,80 F2 segregation plants of Qingchun 533×Nuomai No1 were harvested,four PCR-based markers were used to detect the genotypes of these plants,amylose content determination of these progeny plants with different genotypes was also conducted.The number of plants that lacked of Wx-A1、Wx-B1、Wx-D1 were 8、11、9 respectively;10 plants lacked both Wx-A1 and Wx-D1,12 plants lacked both Wx-B1 and Wx-D1,9 plants lacked both Wx-A1 and Wx-B1,8 plants lacked the three Wx genes.The amylose content was 5.28%~11.35% lower than that of Qingchun 533 while one Wx gene was absent.When two Wx genes were absent in progeny plants,the amylose content was 10.73%~19.81% lower than that of Qingchun 533.The progeny plants with absence of three Wx genes had the lowest amylose content(0.97%).The results of this study were subtly different with the previous report,the decrease of amylose content was more acute when there was an absence of Wx gene(genes) in progeny plants,decrease of amylose content in progeny plants with absence of Wx-B1 was more acute than that of progeny plants with absence of Wx-A1 and Wx-D1.
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Claims 12
Claim · model-written summary
Eight progeny plants lacked Wx-A1.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-A1e0
- Outcome
- plants that lacked of Wx-A1e0
- Value
- 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The number of plants that lacked of Wx-A1、Wx-B1、Wx-D1 were 8、11、9 respectively”
t0“Spring Wheat”
Claim · model-written summary
Eleven progeny plants lacked Wx-B1.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-B1e0
- Outcome
- plants that lacked of Wx-A1、Wx-B1、Wx-D1e0
- Value
- 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The number of plants that lacked of Wx-A1、Wx-B1、Wx-D1 were 8、11、9 respectively”
t0“Spring Wheat”
Claim · model-written summary
Nine progeny plants lacked Wx-D1.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-D1e0
- Outcome
- plants that lacked of Wx-A1、Wx-B1、Wx-D1e0
- Value
- 9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The number of plants that lacked of Wx-A1、Wx-B1、Wx-D1 were 8、11、9 respectively”
t0“Spring Wheat”
Claim · model-written summary
Ten progeny plants lacked both Wx-A1 and Wx-D1.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-A1 and Wx-D1e0
- Outcome
- plants lacked both Wx-A1 and Wx-D1e0
- Value
- 10 plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“10 plants lacked both Wx-A1 and Wx-D1”
t0“Spring Wheat”
Claim · model-written summary
Twelve progeny plants lacked both Wx-B1 and Wx-D1.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-B1 and Wx-D1e0
- Outcome
- plants lacked both Wx-B1 and Wx-D1e0
- Value
- 12 plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“12 plants lacked both Wx-B1 and Wx-D1”
t0“Spring Wheat”
Claim · model-written summary
Nine progeny plants lacked both Wx-A1 and Wx-B1.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-A1 and Wx-B1e0
- Outcome
- plants lacked both Wx-A1 and Wx-B1e0
- Value
- 9 plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“9 plants lacked both Wx-A1 and Wx-B1”
t0“Spring Wheat”
Claim · model-written summary
Eight progeny plants lacked all three Wx genes.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx genese0
- Outcome
- plants lacked the three Wx genese0
- Value
- 8 plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“8 plants lacked the three Wx genes”
t0“Spring Wheat”
Claim · model-written summary
Progeny missing one Wx gene had 5.28%~11.35% lower amylose content than Qingchun 533.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx genee0
- Outcome
- amylose contente0
- Direction
- lowere0
- Value
- 5.28%~11.35%e0
- Comparator
- Qingchun 533e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The amylose content was 5.28%~11.35% lower than that of Qingchun 533 while one Wx gene was absent.”
t0“Spring Wheat”
Claim · model-written summary
Progeny missing two Wx genes had 10.73%~19.81% lower amylose content than Qingchun 533.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx genese0
- Outcome
- amylose contente0
- Direction
- lowere0
- Value
- 10.73%~19.81%e0
- Comparator
- Qingchun 533e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“When two Wx genes were absent in progeny plants,the amylose content was 10.73%~19.81% lower than that of Qingchun 533.”
t0“Spring Wheat”
Claim · model-written summary
Progeny missing three Wx genes had the lowest amylose content, 0.97%.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx genese0
- Outcome
- amylose contente0
- Direction
- loweste0
- Value
- 0.97%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The progeny plants with absence of three Wx genes had the lowest amylose content(0.97%).”
t0“Spring Wheat”
Claim · model-written summary
The amylose decrease with Wx gene absence was more acute than in a previous report.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx gene(genes)e0
- Outcome
- decrease of amylose contente0
- Direction
- more acutee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the decrease of amylose content was more acute when there was an absence of Wx gene(genes) in progeny plants”
t0“Spring Wheat”
Claim · model-written summary
Wx-B1 absence produced a more acute amylose decrease than Wx-A1 or Wx-D1 absence.
- Taxon
- Spring Wheat
Triticum aestivumt0 - Gene
- Wx-B1e0
- Outcome
- decrease of amylose contente0
- Direction
- more acutee0
- Comparator
- Wx-A1 and Wx-D1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“decrease of amylose content in progeny plants with absence of Wx-B1 was more acute than that of progeny plants with absence of Wx-A1 and Wx-D1.”
t0“Spring Wheat”